主成分分析用于清肺消痤凝胶体外经皮渗透研究.docVIP

主成分分析用于清肺消痤凝胶体外经皮渗透的研究 　　[摘要]该文运用主成分分析法建立一种中药复方透皮给药的评价方法，并以此方法优选清肺消痤凝胶的最佳透皮吸收促进剂。实验中以离体小鼠腹部皮肤为透皮屏障，采用改良Franz扩散池装置进行透皮吸收试验，HPLC建立接受液的指纹图谱，确定共有峰，求出各共有峰的面积，应用主成分分析法求得各组不同时间点的总因子得分，并以各因子得分代替药物浓度求得药物累积释放量Q12，算出增渗倍数ER，以此比较各促进剂对药物的透皮促进作用。结果显示，与空白组相比，各促进剂均对药物有促进作用，而以2.5%氮酮+2.5%薄荷醇混合使用时的促进作用最强。主成分分析法可以较全面的反应接受液中药物的信息，以此建立的评价方法可准确的优选出透皮吸收促进剂，并为后续中药复方经皮给药提供了可借鉴的研究思路。 　　[关键词]中药复方;经皮吸收;主成分分析;透皮促进剂 　　In vitro transdermal delivery of Qingfei Xiaocuo gel based on 　　principal component analysis 　　REN Wei-gao， PENG Lin-xiu， LEI Fei-fei， SUN Cheng-xiang， PAN Jin-huo* 　　（Nanjing University of Chinese Medicine， Nanjing 210023， China） 　　[Abstract]The objective of the present study was to establish a method based on principal component analysis （PCA） for the study of transdermal delivery of Chinese medicinal formulae， and to choose the best penetration enhancers for Qingfei Xiaocuo gel depend on this method. Using improved Franz type diffusion cell and excised rat skin in vitro as transdermal barrier， the receptive solution fingerprint was established by HPLC， harvesting the areas of the common peaks in the fingerprint， then the total factor scores of the concentrations at different times were calculated using PCA and were employed instead of the concentrations to compute the cumulative amounts（Q12） and enhancement ratio （ER）， the latter of which were considered as the indexes for optimizing penetration enhancers. Compare to the control group， the ER of the other groups increased significantly and furthermore， 2.5% azone with 2.5% menthol manifested the best effect. PCA represent most information in the receptive solution， the method above could choose the best penetration enhancers， it could be a reference for the study of transdermal delivery of Chinese medicinal formulae. 　　[Key words]Chinese medicinal formulae; transdermal delivery; principal component analysis; penetration enhancers 　　doi：10.4268/cjcm 　　清肺消痤凝胶处方最早源自于《医宗金鉴》之“颠倒散”，在长期的临床实践中经化裁而成。本方主要由大黄、黄芩、硫磺、桑白皮等药味