中药有效成分硫酸多聚糖WSS25在动物胶质瘤血管再生中实验研究.docVIP

中药有效成分硫酸多聚糖WSS25在动物胶质瘤血管再生中的实验研究 　　【摘要】 目的：分析中药有效成分硫酸多聚糖WSS25对动物模型下脑胶质瘤血管再生的影响作用。方法：以10只正常大鼠脑组织作为对照组，以20只C6胶质瘤模型大鼠脑肿瘤组织作为观察组，对比两组微血管密度计数（MVD）及CD34阳性表达率差异。同时将30只C6胶质瘤模型大鼠分为未接受药物干预的未干预组及接受硫酸多聚糖WSS25干预的干预组，每组15只。对比两组MVD计数及CD34阳性表达率差异。结果：观察组脑组织或肿瘤组织中MVD计数及CD34阳性表达率均明?@高于对照组（P0.05），干预组脑肿瘤组织中MVD计数及CD34阳性表达率均明显低于未干预组（P0.05）。结论：大鼠C6胶质瘤模型脑肿瘤组织中血管均明显增生，且经硫酸多聚糖WSS25干预后，脑肿瘤组织中血管可显著降低，其作用机制可能与CD34密切相关。 　　【关键词】 硫酸多聚糖WSS25； 大鼠； 胶质瘤； 血管再生 　　Study the Role of Glioma Angiogenesis and Its Mechanism of Chinese Medicine Effective Component Polysaccharide Sulfate WSS25 in Animal Models of Cerebral Glioma Angiogenesis/XU Ling，WANG Pei，XIE Ming-xiang，et al.//Medical Innovation of China，2017，14（02）：001-004 　　【Abstract】 Objective：To analyze the glioma angiogenesis and its mechanism of component Polysaccharide Sulfate WSS25 impact on animal models of cerebral glioma angiogenesis in animal models of cerebral glioma angiogenesis.Method：As the control group，with 10 normal rats，20 C6 glioma model rats were as the observation group，counting microvascular density（MVD） and CD34 positive expression rate the positive expression rate differences of two groups were compared.At the same time，30 rat of C6 glioma model were divided into the intervention group（Polysaccharide Sulfate WSS25） and the without intervention group，15 cases in each group.MVD counting and CD34 positive expression rate of two groups were compared.Result：MVD and CD34 positive expression in the observation group of brain tissue were significantly higher than those of the control group（P0.05），MVD and CD34 positive expression in the intervention group were lower than those of the without intervention group（P0.05）.Conclusion：Rat C6 glioma model in brain tissue hyperplasia of blood vessels and lymphatic vessels are obvious，and the polysaccharide sulfate WSS25 after the intervention，in the brain blood vessels and lymphatic vessels can be significantly reduced，its mechanism may be closely related with CD34. 　　【Key words】 Polysaccharide