人类免疫缺陷病毒Ⅰ型B亚型感染者人类白细胞抗原Ⅰ类基因对病毒载量影响.docVIP

人类免疫缺陷病毒Ⅰ型B亚型感染者人类白细胞抗原Ⅰ类基因对病毒载量的影响 　　[摘要] 目的 探讨人类免疫缺陷病毒Ⅰ型（HIV-1）B′亚型感染者人类白细胞抗原（HLA）-A、-B、-C位点等位基因的分布及其对病毒载量的影响。 方法 对146例HIV-1B′亚型感染者采用聚合酶链反应-序列特异性引物（PCR-SSP）扩增方法进行HLA-A、-B、-C位点等位基因检测，计算各位点等位基因的频率及其对感染者病毒载量的影响。 结果 在146例HIV-1B′亚型感染者中分别检出HLA-A位点14个等位基因，-B位点24个等位基因，-Cw位点12个等位基因，其中频率大于0.1的等位基因为HLA-A*02，-A*11，-A*24，-A*30，-B*13，-B*40，-B*51，-Cw*03，-Cw*06，-Cw*08；HLA-A、-B、-C等位基因纯合子携带者的病毒载量高于杂合子（P=0.0013）；HLA-A*03（P=0.0314）、-A*30（P=0.0072）、-B*13（P=0.0087）、-Cw*06（P=0.0145）等位基因携带者具有较低病毒载量。 结论 HIV-1B′亚型感染者HLA-A*03、-A*30、-B*13、-Cw*06等位基因与低病毒载量相关。 　　[关键词] 人类免疫缺陷病毒；人类白细胞抗原；病毒载量；感染 　　[中图分类号] R117[文献标识码] A[文章编号] 1673-7210（2014）06（c）-0009-04 　　 　　Impact of HLA class Ⅰ alleles on viral load in HIV-1 B′ infected individuals 　　MA Yongli CHEN Yanli HAO Jinjuan RUAN Yuhua SHAO Yiming HONG Kunxue▲ 　　National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Control and Prevention, Beijing 102206, China 　　[Abstract] Objective To study the distribution of HLA-A, -B, and C alleles in HIV-1 B′infected individuals and to analyze its impact on HIV-1 viral load. Methods 146 HIV-1 infected individuals were recruited and their HLA-A, -B, and C alleles were genotyped using PCR-SSP technique. Allele frequencies and association of HLA-A, -B, and C alleles with HIV-1 viral loads were also analyzed. Results 14 HLA-A allels, 24 HLA-B allels, and 12 HLA-Cw allels were detected in 146 HIV-1 B infected individuals. Of them, HLA-A*02, -A*11, -A*24, -A*30, -B*13, -B*40, -B*51, - Cw*03, -Cw*06, -Cw*08 were among the most common alleles with frequencies above 0.1; subjects homozygous at HLA class Ⅰ loci had higher viral loads (P=0.0013), subjects carrying HLA-A*03 (P=0.0314), -A*30 (P=0.0072), -B*13 (P=0.0087) and -Cw*06 (P=0.0145) alleles had lower viral loads. Conclusion HLA-A*03, -A*30, -B*13 and -Cw*06 alleles may associate with lower viral loads in HIV-1 B′infected individuals. 　　[Key words] Human immunodefici