人类组成型和可变外显子密码子偏性及聚类分析.docVIP

人类组成型和可变外显子的密码子偏性及聚类分析 　　摘要:通过计算人类组成型外显子和可变外显子的RSCU值,对两种外显子的RSCU值进行了比较#65377;结果表明,除了UAG#65380;UGA#65380;UAA 3个终止密码子外,两种外显子的密码子偏性都是相同的#65377;对30条可变外显子和30条组成型外显子序列的RSCU值进行聚类分析,聚类结果不能把两种外显子区别开,证明两种外显子之间的密码子偏性是相同的#65377;对这60条序列的HI进行了聚类分析,聚类结果没有把两种外显子分开#65377;所以外显子序列中没有组成型剪接和可变剪接的信息#65377; 　　关键字:人类;组成型外显子;可变外显子;密码子偏性;聚类 　　中图分类号:Q52文献标识码:A文章编号:0439-8114(2014)11-2675-04 　　 　　Codon Bias and Cluster Analysis of the Constitutive Exons and 　　the Alternative Exons of Human 　　 　　ZHANG Zhan-jun,XING Yong-qiang,WANG Cheng-ai 　　(School of Mathematics, Physics and Biological Engineering,Inner Mogolia University of Science 　　Technology,Baotou 014010, Inner Mogolia,China) 　　 　　Abstract: RSCU of the constitutive exons and alternative exons of human were calculated. Two kinds of exons RSCU values were compared. The results showed that every codon bias was the same in two kinds of exons, except three stop codons(UAG,UGA,UAA). 30 constitutive exon sequences and 30 alternative exon sequences were selected to calculate RSCU values of them. The cluster analysis did not distinguish two kinds of exons, proving that the codon biases were the same between the two kinds of exons. HI of the 60 sequences were calculated and used to cluster analysis. It was not able to separate the two kinds of exons. It is indicated that there is no constitutive splicing and alternative splicing information in the exon sequences. 　　Key words: human;constitutive exons;alternative exons;codon bias;clustering 　　 　　基金项目:国家自然科学基金项目 　　 　　可变剪接是多细胞真核生物蛋白质多样性的主要来源,且由于可变剪接的存在使得剪接机制变得异常复杂[1]#65377;基于深度测序技术,估计超过95%的人类多外显子基因存在可变剪接现象#65377;可变剪接主要包括5种类型:可变5’剪接(Alternative 5’ splicing)#65380;可变3’剪接(Alternative 3’ splicing)#65380;外显子跳跃(Exon skipping)#65380;内含子保留(Intron retention)#65380;互斥外显子(Mutually exclusive exons)#65377;如果把外显子分为两类,可以分为组成型外显子(Constitution exons)和可变外显子(Variable exons)#65377;上述5种外显子均属于可变外显子#65377; 　　密码子具有简并性,即组成蛋白质的每个氨基酸可能由一个到多个密码子编码#65377;在基因中, 同义密码子的使用并不是完全均匀的#65377;在不同物种#65380;不同生物体