低强度脉冲超声刺激对人类牙周膜细胞BMP2表达效应研究.docVIP

低强度脉冲超声刺激对人类牙周膜细胞BMP-2表达效应的研究 　　[摘要]目的：探讨一定强度的LIPUS对H-PDLCs进行辐照后，细胞内BMP-2的表达变化，对LIPUS诱导牙周成骨效应进行初步评估。方法：体外培养H-PDLCs，LIPUS（90mW/cm2，20min/天）连续处理1周，分别于处理1、3、5、7天后收集标本，同期培养的未接受任何处理的H-PDLCs为对照组。实时定量PCR检测各时间点细胞内BMP-2基因表达变化，2^（-△△CT）法分析基因相对表达变化量，对△CT值组间差异进行方差分析。结果：实时定量PCR显示LIPUS处理后H-PDLCs内BMP-2表达逐渐增强，于第3天达高峰，第5天逐渐减弱，但表达仍高于同期未处理组，到第7天下降到接近同期未处理组水平。尤其是，LIPUS处理3天后较同期对照组BMP-2基因表达增加6.07倍；5天后较同期对照组BMP-2基因表达增加2.30倍，△CT 值组间均具有统计学差异（P0.05）。结论：本研究发现LIPUS处理可有效诱导H-PDLCs的BMP-2表达增强，随时间变化呈现一定的规律性。这表明LIPUS具有潜在的促进H-PDLCs成骨分化效应。 　　[关键词]牙周组织缺损；牙周膜细胞；低强度脉冲超声；BMP-2；成骨效应 　　[中图分类号]R781.4[文献标识码]A[文章编号]1008-6455（2011）05-0767-03 　　 　　Effects of Low Intensity Pulsed Ultrasound on the expression of BMP-2 in human periodontal ligament cells 　　YANG Zun1，REN Lei-xi1,SONG Jin-lin1,ZHOU Jie1,LI Fa-qi2,ZHAO Chun-liang2,WANG Zhi-biao2 　　（1.Department of Orthodontics, College of Stomatology, Chongqing Medical University；2 Chongqing Key Laboratory of Ultrasound Medical Engineering） 　　 　　Abstract:Objective To investigate the expression of BMP-2 in human periodontal ligament cells（H-PDLCs） after a certain intensity of Low Intensity Pulsed Ultrasound(LIPUS), and to evaluate the osteogenic effect of LIPUS on the periodontium.MethodsH-PDLCs were cultured in vitro, and stimulated with LIPUS （90 mW/cm2, 20min/day） for one week. The samples were collected on the first day, third day, fifth day, and seventh day after the role of LIPUS. The H-PDLCs were culturedin the same conditions but without LIPUS management as control group. The expression of BMP-2 in H-PDLCs at each time point were detected by Real-time PCR,and the relative gene expression were analyzed by the method of 2^（-△△CT）. Statistical analysis was performed by analysis of variance (ANOVA).ResultsExpression of BMP-2 increased in LIPUS treatment group, reach the peak on day 3,then decreased on day 5, and then dropped to the original level on day 7.Analysis showed that the expression of BMP-2 gene expression increased to 6.07 times on the third day