低温胁迫对小叶朴离体叶片生理生化指标影响.docVIP

低温胁迫对小叶朴离体叶片生理生化指标的影响 　　摘 要：选取1年生小叶朴营养枝的离体叶片为材料，对其进行不同低温（5，0，-5，-10，-15，-20 ℃）胁迫处理，分别测定叶片中的超氧化物歧化酶（SOD）活性和相对电导率，以及丙二醛（MDA）、可溶性蛋白、脯氨酸和可溶性糖含量，研究小叶朴叶片的抗寒性。结果表明，随着胁迫温度的降低，小叶朴离体叶片SOD活性表现出先升高后降低的趋势，至-10 ℃达到最高值；相对电导率和MDA含量表现出一直升高的趋势；可溶性蛋白含量表现出一直降低的趋势；脯氨酸和可溶性糖含量表现出先升高后降低的趋势，亦至-10 ℃达到最高值。综合说明，低温胁迫使小叶朴叶片细胞膜受到伤害，在高于-10 ℃可通过提高渗透调节物质和保护酶活性提高低温抗性，但在低于-10 ℃，渗透调节物质和保护酶活性亦降低。 　　关键词：低温胁迫；小叶朴；生理指标 　　中图分类号：S792.99 文献标识码：A DOI 编码：10.3969/j.issn.1006-6500.2018.05.005 　　Abstract： The experiment was conducted to study the influence of low temperature on Celtis bungeana with the excised leaves of annual vegetative branches . Six low temperature treatments were set， including 5， 0， -5， -10， -15， -20 ℃， the superoxide dismutase （SOD）， electrical conductivity， and also the content of malondialdehyde （MDA）， soluble protein， proline， and soluble sugar were measured after 10 days under treatments. The results showed that： with the decreasing of temperature， SOD of C. bungeana excised leaves increased and then decreased， the maximum was at -10 ℃； the relative conductivity and MDA content were increased continuously， soluble protein content showed a decreasing trend； soluble sugar and proline content increased and then decreased， the maximum were both at -10 ℃. In conclusion， under low temperature stress， C. bungeana leaf membrane was injured， and C. bungeana leaf could increase the cold resistance through promoting the osmotic adjustment substances content and protective enzyme activity when the temperature was higher than -10 ℃， but the osmotic adjustment substances content and protective enzyme activity were decreased when the temperature was lower than -10 ℃. 　　Key words： low temperature stress； Celtis bungeana； physiological index 　　小?~朴（Celtis bungeana）属于榆科朴属落叶乔木，该树叶大、质厚、色浓绿，树形端正，树冠整齐[1]。其具有很强的抗有毒气体能力，耐粉尘和烟尘；加上该树具有优良的种质资源[2]，可作行道树和学校、医院、居民区、厂区绿化树种，也可以利用其乡土树种的优势，进行荒山造林[1]。真树皮纤维可代麻用或作造纸和人造棉原料；木材供建筑用、树干可药用，主治支气管哮喘及慢性气管炎。由此可见，小叶朴具有较高的生态和经济价值。 　　在低温胁迫下，植物生理生化指标会发生变化，主要包括细胞膜相对透性、酶活性，以及脯氨酸和可