全反式维甲酸对胃癌BGC823细胞增值和凋亡影响及其机制研究.docVIP

全反式维甲酸对胃癌BGC823细胞增值和凋亡影响及其机制研究 　　【摘要】 目的 探讨全反式维甲酸(alltrans retinoic acid,ATRA)对体外培养的胃癌BGC823细胞增殖和细胞凋亡的影响。方法 不同浓度全反式维甲酸处理培养的胃癌BGC823细胞后,采用MTT法检测细胞的增殖活性,采用TUNEL法检测细胞凋亡情况,采用RTPCR检测细胞Survivin基因mRNA的变化。结果 全反式维甲酸对胃癌BGC823细胞的生长具有明显的抑制作用,其作用表现为剂量依赖性和时间依赖性(P0.05)。TUNEL检测显示:以5 μmol/L的全反式维甲酸对BGC823细胞处理0、24、48、72 h后细胞凋亡率逐渐增加(P0.05),呈时间依赖性。RTPCR检测显示BGC823细胞Survivin基因的mRNA表达呈时间依赖性下调。结论 全反式维甲酸对胃癌BGC823细胞株具有明显的增殖抑制和诱导凋亡作用,其作用机制可能与下调Survivin基因表达有关。 　　【关键词】胃癌;BGC823;全反式维甲酸;细胞增殖;凋亡;Survivin 　　 　　Effect of ATRA on proliferation and apoptosis of human BGC823 gastric cancer cell 　　SHI Guangyong. Oncology Department, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China 　　【Abstract】 Objective To observe the effect of alltrans retinoic acid(ATRA)on proliferation and apoptosis of human gastric cancer cell line BGC823 and its mechanisms.Methods BGC823 cells were treated with different concentration of ATRA.The cell proliferatiwas measured by methabenzthiazuron(MTT)assay and the apoptosis induced by ATRA was evaluated by TUNEL assay.The Survivin mRNA expression levels were detected by RTPCR.Results ATRA inhibited growth of BGC823 cells in a dosedependent and timedependent manner(P0.05).Cell apoptosis index increased gradually after exposure to ATRA for 24 h,48 h,72 h in a dose dependent manner(1 μmol/L)(P0.05).Survivin mRNA levels of BGC823 cells treated with 1 μmol/L of ATRA were reduced over time.Conclusion ATRA inhibits the proliferation and induces apoptosis in human gastric cancer cell line BGC823.The effect mechanism is related to arrest the expression of Survivin gene. 　　【Key words】Gastric cancer; BGC823; Cell proliferation; Apoptosis; ATRA; Survivin 　　 　　作者单位:450052郑州大学第五附属医院肿瘤科 　　 　　胃癌是消化系统最常见的恶性肿瘤。虽然近年来在其诊治方面取得了一些成绩,但预后仍不理想,我国胃癌的死亡率仍居恶性肿瘤之首。全反式维甲酸(alltrans retinoic acid,ATRA)是肿瘤诱导分化剂,对多种肿瘤细胞有诱导分化和促进凋亡作用[1],但是其具体机制还不是很清楚。本研究应用全反式维甲酸作用于人胃癌BGC823细胞,来探讨其对胃癌细胞增殖和凋亡的影响及其机制,为其临床应用提供参考依据。 　　1 材料与方法 　　1.1 材料与试剂 胃腺癌细胞株BGC823购自中国科学院细胞库。TUNEL原位细胞凋亡检测试剂盒购自洛