化学诱导小鼠先天性肾积水动物模型实验研究.docVIP

化学诱导小鼠先天性肾积水动物模型实验研究 　　【摘要】 目的：探?一种化学诱导小鼠先天性肾积水动物模型的方法。方法：将C57BL/6J孕鼠随机分为实验组和对照组，每组10只。在第12个妊娠日（GD），对照组采用玉米油灌胃，实验组采用2，3，7，8-四氯二苯并二?f英（TCDD）灌胃。GD18，统计胎鼠的数量、存活率以及畸形发生情况。分析胎鼠及孕鼠各主要脏器组织学结构，电镜观察胎鼠输尿管和肾脏的超微结构。结果：GD18，对照组胎鼠的输尿管和肾脏均正常。实验组胎鼠肾积水发生率为100%（3级86.2%，4级13.8%）。实验组胎鼠下段和中段输尿管黏膜层移行上皮增生，管腔狭窄，输尿管上皮细胞核仁明显，靠边，核大，染色质丰富，异染色质少，常染色质发达；肾间质纤维化，肾小管结构模糊，上皮细胞肿胀，膜性结构破裂。对照组胎鼠肾积水发生率低于实验组。结论：TCDD所诱导的胎鼠肾积水动物模型，与人类肾盂输尿管连接处梗阻（UPJO）在病理学表现上类似，可用于UPJO发病机制与病因学等方面的研究。 　　【关键词】 小鼠； 先天性肾积水； 动物模型 　　Study on Animal Model of Congenital Hydronephrosis Induced by Chemical in Mice/LIU Jing，LIU Mingxue，LIU Xing，et al.//Medical Innovation of China，2017，14（35）：033-036 　　【Abstract】 Objective：To explore a kind of chemical method that can induce animal model of congenital hydronephrosis in mice.Method：C57BL/6J dams were randomly divided into the experimental group and the control group，ten mice in each group.Pregnant mice in the control group were given corn oil with intragastric administration，2，3，7，8-tetrachlorodibenzodioxin（TCDD） was given to pregnant mice with same method in the experimental group on gestational day（GD） 12.Teratogeny of fetus was distinguished，number and survival rate of fetus were calculated，main organs of fetus and dams were gain for histology analysis，ultramicrostructure of fetal ureter and kidney was observed with transmission electron microscope（TEM），on GD18.Result：On GD18，fetal ureters and kidneys were normal in the control group，incidence rate of hydronephrosis in fetus were 100%（grade 3 was 86.2%，grade 4 was 13.8%） in the experimental group.Epithelial proliferation in mucous membrane of inferior and middle ureter，narrow ureter lumina was found in hydronephrosis fetus.Renal interstitial fibrosis，epithelial cells of nephric tubule was swollen with vague and break structure， epithelial cells of ureter was shown with gross nucleoli，big nucleus，rich caryotin，few heterochromatin，and prosperous euchromatin which were found by TEM in the experimental group，on GD18. Hydronephrosis in