单向灌流法研究巴戟天低聚糖大鼠肠吸收特性.docVIP

单向灌流法研究巴戟天低聚糖大鼠肠吸收特性 　　[摘要]采用大鼠在体肠单向灌流实验模型，利用HPLC-ELSD测定肠灌流液中蔗糖、蔗果三糖、耐斯糖、1F-果呋喃糖基耐斯糖和巴戟甲素等5种低聚糖的含量，研究各低聚糖在小肠段（十二指肠、空肠、回肠）和结肠的吸收情况，并考察药物浓度、灌流液pH以及P-糖蛋白（P-gp）抑制剂对药物吸收的影响，得出各低聚糖成分在大鼠体内的肠吸收机制。结果显示5种低聚糖均为全肠道吸收的药物，吸收速率与灌流液pH、药物浓度和肠段部位有关。盐酸维拉帕米可显著增加蔗糖与巴戟甲素的吸收量，提示蔗糖与巴戟甲素是P-gp的底物。5种低聚糖在大鼠肠道内的吸收机制以被动扩散方式为主，不存在饱和吸收，其在全肠段的吸收均较好，吸收部位主要在十二指肠和空肠。 　　[关键词]巴戟天低聚糖;肠吸收;单向灌流法;P-糖蛋白 　　[收稿日期]2014-05-14 　　[基金项目]国家自然科学基金项目;东莞市医疗卫生科技计划一般项目（2014105101295）;广东医学院科研基金博士学位人员科研启动项目（B2013007） 　　[通信作者]*林励，研究员，博士生导师，研究方向为中药资源开发利用与新药研究，Tel：（020E-mail：LL76611@126.com 　　[作者简介]邓少东，讲师，博士，研究方向为中药资源开发利用与新药研究，E-mail：donggdyxy@163.com 　　Study on intestinal absorption features of oligosaccharides in 　　Morinda officinalis How.with sigle-pass perfusion 　　DENG Shao-dong1， ZHANG Peng2， LIN Li2*， XIAO Feng-xia2， LIN Jing-ran2 　　（1.The Second School of Clinical Medicine， Guangdong Medical College， Dongguan 523808， China; 　　2. College of Chinese Materia Medica， Guangzhou University of Traditional Chinese Medicine， Guangzhou 510006， China） 　　[Abstract]To study the in situ intestinal absorption of five oligosaccharides contained in Morinda officinalis How. （sucrose， kestose， nystose， 1F-Fructofuranosyinystose and Bajijiasu）. The absorption of the five oligosaccharides in small intestine （duodenum， jejunum and ileum） and colon of rats and their contents were investigated by using in situ single-pass perfusion model and HPLC-ELSD. The effects of drug concentration， pH in perfusate and P-glycoprotein inhibitor on the intestinal absorption were investigated to define the intestinal absorption mechanism of the five oligosaccharides in rats. According to the results， all of the five oligosaccharides were absorbed in the whole intestine， and their absorption rates were affected by the pH of the perfusion solution， drug concentration and intestinal segments. Verapamil Hydrochloride could significantly increase the absorptive amount of sucrose and Bajijiasu， suggesting sucrose and Bajijiasu are