四种生长素对刺梨离体枝条繁殖生根影响.docVIP

四种生长素对刺梨离体枝条繁殖生根影响 　　摘要：为了研究4种生长素对刺梨（Rosa roxbunghii Tratt）离体枝条生根的影响，建立刺梨简单高效离体繁殖技术，利用不同浓度（10、30、70、90 mg/L）的萘乙酸、赤霉素、吲哚乙酸和吲哚丁酸溶液，分别对一年生、二年生、多年生等3个时期的刺梨枝条进行离体处理，在室温（20～25 ℃）下培养15 d后测定其在不同浓度的4种生长素处理下的生根情况。结果表明，不同时期刺梨枝条在不同激素溶液及同一激素不同浓度中的生根率均不相同。其中，二年生与多年生枝条生根率极显著高于一年生枝条（P0.01）；吲哚乙酸与吲哚丁酸对刺梨两年生与多年生离体枝条生根效应较好。10～70 mg/L的吲哚丁酸和吲哚乙酸对刺梨枝条的生根效应显著，生根率高、生根数较多，移栽土中的成活率较高，生长良好。结合再生苗与移栽生长情况，刺梨离体再生繁殖应选用二年生离体枝条吲哚乙酸溶液50 mg/L处理。 　　关键词：刺梨（Rosa roxbunghii Tratt）；离体繁殖；生根；生长素 　　中图分类号：S661.2 文献标识码：A 文章编号：0439-8114（2014）02-0441-03 　　Effects of Four Growth Hormones on Branch Cuttage of Thorn Pear 　　（Rosa roxburghii Tratt） 　　WANG Lin-jiao1a，SHENG Mao-yin1b，2 　　（1a.School of Vocation and Technology； 1b.Institute of South China Karst， Guizhou Normal University， Guiyang 550001， China； 2.The State Key Laboratory Incubation Base for Karst Mountain Ecology Environment of Guizhou Province， Guiyang 550001， China） 　　Abstract： Effects of four growth hormones on branch cuttage were studied and the simple and efficient technique for the in vitro propagation of thorn pear was developed. The annual， biennial and perennial in vitro branches of thorn pear were treated in different doses（10， 30， 50， 70， 90 mg/L） of NAA， GA3， IAA， and IBA， respectively. The rootage ratios were calculated after 15 days cultivating in room temperature（20～25 ℃）. The results showed that the rootage ability of biennial and perennial braches was significantly stronger than that of annual branches（P0.01）. The rootage ratios of branches treated by IAA and IBA are significantly higher than that treatedly NAA and GA3. Especially， the 10～70 mg/L concentration of IBA and IAA had higher effect of rootage on branches at all three age stages than others. The regrowth survive form raised more easily. The studies indicated that the in vitro propagation of thorn pear could use the biennial branches treated by 50 mg/L IAA. 　　Key words： Rosa roxburghii Tratt； in vitro propagation； rooting； growth hormone 　　刺梨（Rosa roxbunghii Tratt）是蔷薇科多