基于UPLCQTOFMSE技术彝药满山香化学成分鉴别研究.docVIP

基于UPLCQTOFMSE技术彝药满山香化学成分鉴别研究 　　[摘要]利用超高效液相色谱串联四极杆飞行时间质谱技术（UPLCQTOF/MSE），对彝药满山香的化学成分进行在线快速分析鉴定。以水乙腈为流动相，梯度洗脱，ESI 正、负离子模式下采集数据，通过UNIFI数据库检索并人工复核相对分子质量、特征离子、中性丢失、色谱保留时间等信息，并结合对照品信息和文献报道等对其进行验证；对于未知成分，根据其精确相对分子质量、质谱裂解碎片、色谱保留行为以及已知同类化合物的特征碎片，对其进行结构推测，确定化学成分的结构。该研究共鉴定出包括原花青素B2、南五味子酸、五味子酯甲、异形南五味子丁素和戈米辛及其衍生物等68个化合物的结构，其中有37个首次在满山香中发现的化合物，1个待确定化合物。 　　[关键词]满山香； 化学成分； 鉴别； UPLCQTOF/MSE； UNIFI数据库 　　[Abstract]This study was to identify the chemical constituents of Schisandra propinqua， one herbal medicine of Yi nationality in China by using ultra performance liquid chromatographyquadrupoletime of flightmass spectrometry （UPLCQTOF/MSE） Acetonitrile water containing 01% formic acid was used as the mobile phase for gradient elution Data were collected under ESI negative mode and ESI positive mode， and then screened and verified by the software of UNIFI and Masslynx41 Based on the accurate mass， fragment ions， neutral losses， mass error， retention time， reference substance， isotope information， the intensity of fragments， as well as the previous reports， the known compounds were validated and identified The chemical structures of the unknown components were identified according to exact molecular weight， MS fragment， chromatographic retention behavior， and characteristic fragments of known congener compounds A total of 68 chemical components were identified from S propinqua， including 3 flavonoids， 10 flavanols， 34 lignans compounds （including 20 dibenzocyclooctene lignans）， 4 triterpenoids， 17 organic acid and other compounds 37 compounds of them were found in S propinqua for the first time， and one potential compound needed to be identified. 　　[Key words]Schisandra propinqua； Chemical constituents； identification； UPLCQTOF/MSE； UNIFI database 　　?M山香来源于木兰科植物合蕊五味子的干燥藤茎和根，为彝族常用药材，用于治疗骨折、慢性胃炎、风湿性关节炎、痛经、外伤出血等。《明代彝医书》记载满山香主要作用于疟疾。满山香根茎提取物的复方注射液临床用于治疗肺癌，以满山香为原料的灯银脑通胶囊用于中风中经络、瘀血阻络的治疗。目前，地方药材标准及彝药文献[13]中没有规定满山香质控的化学成分，也未见有对其整体化学成分研究的报道。满山香药材中仅报道了gomisin，kadsulignan L，intermedia A，五味子丙