基于DNA条形码中药材防风与其混伪品鉴定.docVIP

基于DNA条形码中药材防风与其混伪品鉴定 　　[摘要] 目的 通过ITS2序列分析，对防风及其易混伪品进行鉴定，确保用药安全。 方法 从GenBank数据库下载中药材防风与其混伪品的DNA条形码ITS2序列，利用MEGA 6.06软件进行比对分析，计算GC含量及变异位点、种内、种间遗传距离，并构建NJ系统发育树，应用ITS2数据库网站预测其ITS2二级结构。 结果 序列分析与遗传距离结果显示，防风与其混伪品存在明显差异，基于ITS2序列的NJ树及其ITS2二级结构均可以将防风与其混伪品进行区分。 结论 ITS2能够准确鉴定防风及其易混伪品，可用于中药材的快速鉴别。 　　[关键词] 防风；DNA条形码；混伪品；ITS2；分子鉴定 　　[中图分类号] R927 [文献标识码] A [文章编号] 1674-4721（2016）05（c）-0004-04 　　Identification of Saposhnikovia divaricata and its adulterants based on DNA barcode （ITS2） 　　LIN Hao1 QIAN Jie-ying2 LI Si-lu3 OU Hui-hong1 XIA Hai-shan1，4 LIU Yong1，4▲ 　　1.School of Pharmac，Guangdong Medical University，Dongguan 523808，China；2.Department of Biochemistry and Molecular Biology，Guangxi Medical University，Nanning 530021，China；3.School of Basic Medicine，Guangdong Medical University，Dongguan 523808，China；4.Guangdong Key Laboratory for Research and Development of Natural Drugs，Zhanjiang 524023，China 　　[Abstract] Objective To identify Saposhnikovia divaricata and its adulterants by using ITS2 sequence analysis and to secure medication safety. Methods ITS2 sequences of Saposhnikovia divaricata and its adulterants were downloaded from GenBank，MEGA 6.06 software was used for sequence analysis to summarize GC content，variation sites，estimate intra-and inter-specific genetic distances，and built NJ trees.Moreover，the secondary structure of ITS2 was predicted by using the ITS2 database websites. Results ITS2 sequence analysis and genetic distances calculation showed significant difference in the samples.Further more，NJ tree and secondary structure results could distinctly differentiate quality product and adulterants. Conclusion ITS2 can effectively identify Saposhnikovia divaricata and its adulterants，as a result，it can be used for rapid identification of Chinese medicinal materials. 　　[Key words] Saposhnikovia divaricata；DNA barcoding；Adulterants；Internal transcribed spacer 2；Molecular identification 　　防风（Saposhnikovia divaricata）是双子叶植物伞形科（Umbelliferae）多年生草本植物防风属（Saposhnikovia）防