壳聚糖絮凝澄清工艺与醇沉工艺对党参水提液影响.docVIP

壳聚糖絮凝澄清工艺与醇沉工艺对党参水提液影响 　　摘要：目的 探索壳聚糖絮凝澄清工艺与醇沉工艺对党参水提液中主要化学成分的影响。方法 以党参炔苷含量、浸膏得率及HPLC指纹图谱各组分的相对表观含量作为评价指标，考察壳聚糖絮凝澄清工艺与醇沉工艺2种纯化工艺对党参水提液的影响。结果 壳聚糖絮凝澄清工艺处理的党参水提液其浸膏得率明显高于醇沉工艺，且对极性较强组分的保留效果显著优于醇沉工艺；醇沉工艺对党参炔苷保留量稍高于壳聚糖絮凝澄清工艺，且对极性较弱组分的保留效果较好。结论 上述2种工艺对党参水提液中主要化学成分的影响存在一定的规律，可为党参及其他中药水提液纯化工艺的合理选择提供参考。 　　关键词：党参；纯化工艺；党参炔苷；指纹图谱；相对表观含量 　　DOI：10.3969/j.issn.1005-5304.2017.12.020 　　中图分类号：R284.2 文献标识码：A 文章编号：1005-5304（2017）12-0081-04 　　Effects of Chitosan Flocculation Clarification Process and Alcohol Precipitation Process on Water Extract of Codonopsis Radix PENG Ying， LEI Chang， TANG Ying-nan， ZHOU Li-li， XIA Xin-hua （School of Pharmacy， Hunan University of Chinese Medicine， Changsha 410208， China） 　　Abstract： Objective To explore the effects of chitosan flocculation clarification process and alcohol precipitation process on the principal chemical constituents of water extract of Codonopsis Radix. Methods The influence of two purification processes on water extract of Codonopsis Radix was investigated through lobetyolin contents， extract yield， and relative apparent content of each component in HPLC fingerprint as evaluation indexes. Results Chitosan flocculation clarification process showed a significantly higher extract yield of water extract compared with alcohol precipitation process， and it has a markedly better retention effect for strong polarity constituents； alcohol precipitation process exhibit a little better retention effect for lobetyolin and a better retention effect for weak polarity constituents. Conclusion The above two processes have some regularity in the influence on the main chemical constituents in the water extract of Codonopsis Radix， which can provide some guidance for the reasonable choice of the purification process for water extracts of Codonopsis Radix， and other TCM water extracts. 　　Key words： Codonopsis Radix； purification process； lobetyolin； fingerprints； relative apparent content 　　中药制剂生产常需要采用适合的方法对中药水提液进行纯化处理，以达到去粗取精、提高疗效、减少用药量等目的。近年来，