复合大孔聚多糖止血材料制备及其体外性能研究.docVIP

复合大孔聚多糖止血材料制备及其体外性能研究 　　[摘要] 目的 初步评价复合大孔聚多糖止血材料的止血效果，研究其体外性能。 方法 辐照交联法制备复合大孔聚多糖止血材料，通过红外及理化测试表征材料的结构、降解性能及凝血性能。 结果 止血材料经辐照后分子发生交联，吸水率达3456%；体外降解实验表明，止血材料降解性能良好，在溶菌酶溶液中3 d即可降解68%，20 d内则降解完全；材料的体外凝血时间为（25.33±2.45）s，能吸附更多的红细胞和血小板，血凝块强度更大。 结论 辐照交联技术制备的复合大孔聚多糖止血材料具有较好的吸液性能和凝血性能，在伤口止血方面具有良好的应用前景。 　　[关键词] 辐照交联；羧甲基壳聚糖；止血材料；体外降解；凝血 　　[中图分类号] R318.08 [文献标识码] A [文章编号] 1673-7210（2018）04（b）-0017-04 　　Preparation of the macroporous polysaccharide hemostatic complex materials and study on its performance in vitro 　　XU Ling1 MA Xiaochun1 ZENG Lijun2 CHEN Miaoyuan2 WANG Yun2 JU Xuecheng1 QIU Ximei1 　　1.Peking University Shenzhen Institute， Guangdong Province， Shenzhen 518057， China； 2.Xiamen Gelfeel Biological Technology Co.， Ltd.， Fujian Province， Xiamen 361026， China 　　[Abstract] Objective To evaluate the hemostatic effect of the macroporous polysaccharide hemostasis complex materials preliminarily， and to investigate the performance of the materials in vitro. Methods The hemostatic materials were prepared by γ-irradiation crosslinked method， and its structure， degradation in vitro and clotting performance were characterized by infrared spectrum and physicochemical test. Results The results showed that the crosslinked carboxymethylated-chitosan molecules had been made after the materials were irradiated， and its water absorption was of 3456%. The biodegradable degradation of the materials in lysozyme solution was 68% after 3 days and the degradation completed within 20 days. The clotting time was （25.33±2.45） s， which indicated the material adhered more red blood cells and platelets and showed the stronger blood clots. Conclusion The macroporous polysaccharide hemostasis complex materials prepared by γ-irradiation crosslinked method possess great solution absorption performance and blood coagulation performance， which has good application prospect in wound hemostasis. 　　[Key words] Radiation cross-linking： Carboxymethyl chitosan； Hemostatic material； Degradation in vitro；