STAT3在小鼠胚胎神经系统发育中的表达及与NTD的相关性研究-人体解剖与组织胚胎学专业论文.docxVIP

第三军医大学硕士学位论文 第三军医大学硕士学位论文 PAGE PAGE 1 英文缩写词表 英文缩写 英文全称 中文全称 FGF fibroblast growth factor 成纤维细胞生长因子 FGFR fibroblast growth factor receptor 成纤维细胞生长因子受体 JAK Janus tyrosine kinase Janus 酪氨酸激酶 STAT signal transducer and activator of transcription factor 信号转导与转录激活因子 RA retinoic acid 维甲酸 SDS sodium dodecyl sulfate 十二烷基磺酸钠 CNS central nervous system 中枢神经系统 E(d) Embryonic (day) 胚胎(天数) ISH in situ hybridization 原位杂交 ICC immunocytochemistry 免疫细胞化学 PBS phosphate buffered saline 磷酸盐缓冲液 PCR polymerase chain reaction 聚合酶链式反应 NTD neural tube defect 神经管缺陷 DEPC diethylprocarbonate 二乙基焦碳酸酯 rpm revolution per minute 每分钟转速 SABC streptavidin-biotin complex 链酶亲和素-生物素复合物 FITC fluorescein isothiocyanate 异硫氰酸荧光素 HRP horseradish peroxidese 辣根过氧化物酶 DAB Diaminobenzidine 3,3-二氨基联苯胺 NBT Nitro-Blue-Tetrazolium 四唑氮蓝 BCIP 5-bromo-4-chloro-3-indolyl-phosphate 5-溴-4-氯-3-吲哚-磷酸盐 The expression of STAT3 in the developing nervous system of mouse and the relationship with the NTD Abstract The development of neural tube is an important embryologic event involved in the primordium establishment of the central nervous system. During this process, nervous system can’ t develop normally until the neural plate closes on time accurately, or the neural tube defect (NTD) would occur. Nowadays, the studies of the developmental neurobiology have focused on the molecular mechanisms of the development of nervous system. Some studies have revealed that signal transducer and activator of transcription factor-3 (STAT3) was expressed in the developing brain area strongly at embryo 9.5d (E9.5d), when neural tube closed. Could it be possibe that STAT3 may be involved in the closure of neural tube and its changes were related with NTD? Following the closure, neuroepithelium continued to prolife, differentiate and migrate, and prosencephalon, mesencephalons and rhombencephalon appeared in the anterior region of neural tube. The proliferation, differentiation and migration of the neuroepithelium are essential for the struct