Wnt7a基因对大鼠骨髓间充质干细胞增殖及向神经元样细胞分化影响的分析-外科学(骨外)专业论文.docxVIP

第三军医大学硕士学位论文 第三军医大学硕士学位论文 PAGE PAGE 1 缩略语表 英文缩写 Wnt7a 英文全称 wingless-type MMTV integration 中文全称 无翅型 MMTV 整 site family, member 7A 合位点家族成员7 MSCs mesenchymal stem cells 间充质干细胞 MOI multiplicity of infection 感染复数 PCR Polymerase Chain Reaction 聚合酶链式反应 Ad Adenovirus 腺病毒 FBS fetal calf serum 胎牛血清 DMEM dulbeccos modified eagle medium 细胞培养基 DMSO Dimethyl sulfoxide 二甲基亚砜 LB Luria-Bertani 溶菌肉汤 BSA Albumin from bovine serum 牛血清白蛋白 BCA bicinchonininc acid 二辛可酸 bFGF basic fibroblast growth factor 碱性成纤维生长因子 NSE Neuron Specific Enolase 神经元特异性烯醇化酶 MTT 3-(4,5)-dimethylthiahiazo (-z-y1)- 3-(4，5-二甲基噻唑-2)-2， 3,5-di-phenytetrazoliumromide 5-二苯基四氮唑溴盐 PBS Phosphate Buffer Solution 磷酸盐缓冲液 β-catenin Beta-catenin β 连环素蛋白 SDF-1 stromal cellderived factor 基质细胞衍生因子 CXCR4 Cxc Chemokin Receptor 4 基质细胞衍生因子受体 SD Sprague-Dawley Rat SD 大鼠 SCI spinal cord injury 脊髓损伤 Effect of Wnt7a gene on rat bone marrow mesenchymal stem cell proliferation and differentiation into neuron-like cells Abstract Background： At present, It is still very difficult to treat the spinal cord injury ,mainly because of limited ability to self - repair of nerve tissue. According to literatures, the embryonic stem cells and neural stem cells can be used as seed cells to repair the nerve tissue, however, its source is limited, and it is restricted by ethics. In recent years, with the potential of multi - directional differentiation of bone marrow mesenchymal stem cells (mesenchymal stem cells, MSCs) due to the ease of access and is widely used in cell transplantation for repair of the injured spinal cord, but the lack of neurotrophic factor secretion and environmental induction in the injured spinal cord damage zone, lead to repair effect is not ideal. Therefore, to maintain the long -term survival of the MSCs, improve its differentiation rate to neural cells is very important. The Wnt family play an important role in tumor, muscle regeneration, repair of nervous system, reproductive system, dorsal and ventral limb formation and