“肠病及肺”的病理变化及相关调控物质和ERK信号通路研究中医临床基础专业论文.docx

成都中医药大学博士学位论文 成都中医药大学博士学位论文 PAGE PAGE 10 establishing the rat constipation model. The immunohistochemistry research showed that the positive cells of SP, VIP, CCK8 and CGRP in rat colon and lung tissue were colored brown in HYPERLINK /dict_result.aspx?searchword=%e8%83%9e%e6%b5%86amp;tjType=sentenceamp;styleamp;t=cytoplasm cytoplasm. The research with electron microscope showed that the cell nucleus became pycnosis, the mitochondria were swelled and the crista were disordered with arrangement, the rough endoplasmic reticulum and ribosome were reduced in number and the microvilli became sparse and disarray. At the same time, the HYPERLINK /dict_result.aspx?searchword=%e7%bb%93%e8%82%a0%e7%bb%84%e7%bb%87amp;tjType=sentenceamp;styleamp;t=colon%2Btissue lung tissue of model rat showed that theⅠor Ⅱ aleolar epithelial type cells were swelled, there were some limitations translucent zone and mitochondria were swelled and disordered with arrangement in cell plasma. The third was about the express changes of some controlling substances such as SP, VIP, CCK8 and CGRP and the ERK signaling pathway when the phenomenon of the pathological changes from large intestine to lung was occurred in the rat constipation model. The experimental results showed that the express of P substans was reduced markedly in model rat colon than that in control group (P0.05), at the same time, the express of P substans in model rat lung was risen markedly than that in control (P0.05). The express of VIP was risen markedly in model rat colon than that in control (P0.05), at the same time, the express of VIP in model rat lung was reduced significantly than that in control (P0.01). The express of CCK8 was risen significantly in model rat colon than that in control (P0.01), at the same time, the express of CCK8 in model rat lung was reduced significantly (P0.01). The express of CGRP was risen markedly in model rat colon than that in control (P0.05), at the same time, the express of CGRP in model ra