β-羟基异戊酰紫草素对人卵巢癌细胞株SKOV3体外作用及其机制的实验研究-医学专业论文.docxVIP

万方数据 万方数据 A Study on Antitumor Effect of β-HIVS And Its Mechanism in Human Ovarian Cancer Cell Line Skov3 in Vitro ABSTRACT Objective：To observe the ovarian cancer cell line SKOV3 intervened by β-HIVS and its antitumor mechanism in vitro. Methods：Methyl thiazolyl tetrazolium(MTT) assay was used to establish the dose-effect curves and the synergetic effect of β-HIVS and cisplatin. The morphological changes of the cell were observed by transmission electron microscope in order to evaluate cell apoptosis.The flow cytometry (FCM) was used to observe the apoptosis rate.The expression of Bcl-2 and Bax was detected by Western blotting. Result：The growth inhibition of SKOV3 by β-HIVS was dose-dependent and 50% inhibition rate is 7.37 uM/L. β-HIVS combining with cisplatin obviously increased the inhibitoty rate and apoptosis rate of SKOV3 than those of the corresponding individual cisplatin groups(P0.05).Within a certain concentration range, the synergenic effect of β-HIVS and cisplatin were significantly different in dose-dependnt manner.After 6 hours of exposure to β-HIVS, SKOV3 cell line presented some morphological features of apoptosis,including cell shrinkage. The flow cytometry (FCM) showed the upward tendency of apoptosis rate in experimental groups.Western blotting presented Bcl-2 was declined and Bax was increased by dose of β-HIVS. Conclisions：β-HIVS can inhibit growth of SKOV3 in vitro and induced apoptosis of it. The synergetic effect of β-HIVS and cisplatin is dose-dependnt . Subject words β-HIVS ，ovarian cancer，growth inhibition，apoptosis，concomitant treatment 5 前 言 卵巢癌属中医的“症瘕”、“积聚”、“肠覃”范畴。是女性常见的恶性肿瘤之一，占 女性生殖道恶性肿瘤的第三位，其病因尚不清楚，组织类型繁多复杂，极易发生腹腔转 移直接影响患者的寿命及生存质量。目前卵巢恶性肿瘤的化疗方案是以紫杉醇和铂类为 基础的联合化疗，但肿瘤对化疗药物的耐药性及铂类的毒性，制约着卵巢恶性肿瘤的化 疗效果，使卵巢恶性肿瘤患者的存活率降低，至今，卵巢癌的五年存活率较低徘徊在 25-30%。 紫草是我国传统中药，具有清热、解毒之功效。千百年来被用于治疗斑疹、麻疹、 咽喉痛，痈以及烧伤。近年来，中医临床研究证明，紫草对于治疗癌症特别是肝癌和肺 癌具有较好的效果。β-羟基异戊酰紫草素（简称：β-HIVS）是紫草素脂溶性很强的有 效化学成分，具有多方面的药用价值,尤其在抗肿瘤作用方面。体外实验证实紫草素抑 制多种肿瘤细胞的生长,且呈时间和剂量依赖性,研究主要集中在下列人类肿瘤细胞: