“肺病及肠”病理变化及相关调控物质和ERK信号通路研究中医临床基础专业论文.docx

成都中医药大学博士学位论文 成都中医药大学博士学位论文 “肺病及肠”的病理变化及相关调控物质和 “肺病及肠”的病理变化及相关调控物质和 ERK 信号通路研究 PAGE PAGE 13 PAGE PAGE 10 ABSTRACT Objective：Observed pulmonary disease (allergic asthma) the impact of the large intestine in rats to explore the lung and intestinal mechanisms of pathological changes and related material basis and ERK signaling pathways. Methods：Male Wistar rats were used, a blank group, lung (allergic asthma) group 2 group. In addition to the control group, the lung disease group with 4 points abdominal injection with 1% egg albumin, aluminum hydroxide, saline gel preparation of sensitization, after injection of 1% OVA inhalation ultrasound induced asthma. Pulmonary function test animals a week after atomization; collection of bronchoalveolar lavage fluid and lung intestinal microflora stool; collecting animals and femoral artery blood test blood TNF-α, IL-1; light microscopy lung, duodenum, Jejunum, ileum, colon, rectum, stomach pathologic changes; electron microscopy of lung and colon; immunohistochemistry lung, duodenum, jejunum, ileum, colon, rectum, stomach tissue CCK8, CGRP, SP, VIP Content; RT-PCR detection of lung, colon tissue iNOS, ERK mRNA expression. Results： Pathophysiology Lung functions: lung disease group were significantly increased respiratory frequency, tidal volume, minute ventilation decreased significantly compared with the control group significantly (P 0.01); Stool, texture, wet and dry weight observed: lung solution will reduce the number of rats, defecation difficult than the control group, the appearance of feces smaller, dry and hard texture, grain number reduction, reducing both wet and dry weight (P 0.05); Gastric emptying and intestinal propulsion test: compared with the control group, lung rats increased gastric residual rate (P 0.05), the end of the colon promote the rate of carbon reduction (P 0.05); Pulmonary intestinal microflora: aerobic pulmonary tuberculosis group, fungi increased significantly, anaerobic bacteria were sig