左归降糖解郁方对糖尿病并发抑郁症大鼠海马谷氨酸及N―甲基―D―天冬氨酸受体2A2B影响.docVIP

左归降糖解郁方对糖尿病并发抑郁症大鼠海马谷氨酸及N―甲基―D―天冬氨酸受体2A2B影响 　　摘要：目的 观察左归降糖解郁方对糖尿病并发抑郁症大鼠海马谷氨酸、N-甲基-D-天冬氨酸受体（NMDA receptor，NR）2A及NR2B表达的影响，探讨其对糖尿病并发抑郁症大鼠海马损伤的保护机制。方法 建立糖尿病并发抑郁症大鼠模型，随机分为模型组、阳性药组及左归降糖解郁方高、中、低剂量组，以正常大鼠为正常组，每组16只，各给药组给予相应药物灌胃，连续28 d。采用Open-field实验评价大鼠行为变化，ELISA检测海马谷氨酸含量，免疫荧光法检测海马NR2A、NR2B表达。结果 与正常组比较，模型组大鼠自主活动次数明显减少（P0.01），海马谷氨酸含量明显升高（P0.01），NR2A、NR2B表达明显升高（P0.01）；与模型组比较，阳性药组和左归降糖解郁方高剂量组大鼠自主活动次数明显增加（P0.01），海马谷氨酸含量明显下降（P0.01）， NR2A、NR2B表达降低（P0.05）。结论 左归降糖解郁方可明显改善糖尿病并发抑郁症大鼠的抑郁行为，其药效作用可能与调控大鼠海马谷氨酸含量及NR2A、NR2B表达有关。 　　关键词：左归降糖解郁方；糖尿病；抑郁症；谷氨酸；N-甲基-D-天冬氨酸受体2A；N-甲基-D-天冬氨酸受体2B；大鼠 　　DOI：10.3969/j.issn.1005-5304.2015.10.020 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2015）10-0070-04 　　Abstract：Objective To observe the effect of Zuogui Jiangtang Jieyu Formula on the expressions of glutamate， NR2A and NR2B in hippocampus of diabetic rats with depression；To explore the mechanism of protective effect. Methods Diabetes with depression rat models were established and then were randomly divided into the model group， positive medicine group， high-， medium- and low-dose Zuogui Jiangtang Jieyu Formula groups. Normal rats acted as normal group， 16 rats per group. After 28 days of administration， Open-field test was used to detect the behavior of the rats；glutamate content of hippocampus was detected by ELISA；the expressions of NR2A and NR2B in rat hippocampus were detected by immunofluorescence. Results Compared with normal group， automatic activity times of rats in model group decreased significantly （P0.01）；both glutamate content （P0.01） and expressions of NR2A， NR2B （P0.01） increased significantly. Compared with the model group， automatic activity times of rats in positive medicine group and high-dose Zuogui Jiangtang Jieyu Formula group significantly increased （P0.01）；glutamate content dropped （P0.01）；expressions of NR2A and NR2B decreased （P0.05）. Conclusion Zuogui Jiangtang Jieyu Formula can improve depressive behavior of diabetic rats with depression， which may be related to the regul