出生后早期接触狄氏剂对小鼠神经发育毒性分析-toxicity analysis of early exposure to dieldrin to mouse neurodevelopment after birth.docxVIP

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出生后早期接触狄氏剂对小鼠神经发育毒性分析-toxicity analysis of early exposure to dieldrin to mouse neurodevelopment after birth.docx

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出生后早期接触狄氏剂对小鼠神经发育毒性分析-toxicity analysis of early exposure to dieldrin to mouse neurodevelopment after birth

安徽医科大学硕士学位论文安徽医科大学硕士学位论文 5 5 were evaluated, and a battery of tasks i.e. hole-board test, spontaneous locomotor apparatus, beam walking, open field, Morris water maze (MWM), was used to assess the spontaneous motor activity, sensorimotor activity, anxiety and spatial learning and memory ability in different time. Mice were sacrificed in PND36 and PND98, substantia nigra striatum was separated from brain. The protein expression of GFAP was observed by Western blotting. Results There were trends toward decreased vaginal opening time and delayed testicular descent time. Dieldrin-exposure induced reversible effects on sensorimotor activity which were measured by negative geotaxis test (PND 1) and horizontal bar task. Dieldrin-exposure did not alter mice behavior in open-field, but dieldrin-exposure decreased number of platform-site crossovers in probe trial of MWM test compared to DMSO control. Dieldrin-exposure induced the increasing GFAP’s expression. Conclusions Dieldrin-exposure induced reversible effects on sensorimotor activity which were measured by negative geotaxis test and horizontal bar task , but dieldrin-exposure decreased number of platform-site crossovers in probe trial of MWM test compared to DMSO control. The results suggest that early postnatal exposure to dieldrin affects spatial memory in adult mice. Limited times of early postnatal exposure to dieldrin increase the expression of GFAP in the nigrostriatal systerm. Key words GFAP Dieldrin /Mouse / Neurobehavior /Morris Water Maze/Learning and memory/ 1 1 英文缩略词表英文缩写 ANOVA 英文全称 Analysis of variance 中文全称方差分析 CNS central nervous system 中枢神经系统 DMSO Dimethyl Sulphoxide 二甲基亚枫 EDTA Ethylenediaminetetra-aceticacid 乙二胺四乙酸 Fig Figure 统计图 GFAP glial fibrillary acidic protein 胶质纤维酸性蛋白 GD gestational day 孕…天 ip intraperitoneal injection 腹腔注射 LD50 MWM Median lethal dose Morris water maze 半数致死剂量 Morris 水迷宫 OD Optical density 光密度 P Probability 概率 PBS Phosphatic buffer solution 磷酸盐缓冲盐溶液 PND postnatal day… 出生后…天 r C