当归对阴虚哮喘Balbc小鼠Th1Th2及Th17Treg影响及机制研究.docVIP

当归对阴虚哮喘Balbc小鼠Th1Th2及Th17Treg影响及机制研究 　　[摘要] 当归是临床常用中药，具有补阴养血、活血润燥的功能。为研究当归对阴虚哮喘Balb/c小鼠Th1/Th2及Th17/Treg的影响及机制，该实验通过卵清白蛋白加甲状腺素复制阴虚哮喘Balb/c小鼠模型，造模同时分别给予地塞米松（DXM）、当归提取液、当归提取液+DXM灌胃处理，HE染色进行肺组织病理学检查，ELISA法检测支气管肺泡灌洗液（BALF）中IL-4，IL-17，IFN-γ，TGF-β水平及维甲酸受体相关孤儿受体（RORγt）的表达情况。结果显示，当归可显著减轻肺组织炎症浸润程度，升高IFN-γ/IL-4及TGF-β/IL-17的比值，降低肺组织中RORγt的表达强度，当归与DXM配伍的治疗效果最为显著。提示，当归可通过抑制肺组织RORγt表达、调节Th1/Th2及Th17/Treg的功能平衡，改善气道的炎症状态，发挥对阴虚型哮喘的治疗作用。 　　[关键词] 当归；阴虚哮喘；Balb/c小鼠；Th1/Th2；Th17/Treg 　　[Abstract] Angelicae Sinensis Radix， with nourishing Yin， promoting blood circulation， and moisturizing dryness functions， is commonly used in clinical medicine. In order to investigate the effects and mechanism of Angelica sinensis（AS） on Th1/Th2 and Th17/Treg in mice with asthma and Yin deficiency syndrome， asthmatic and Yin deficiency syndrome Balb/c mice models were established by injecting and inhaling ovalbumin（OVA） and thyroxin. The models were treated with dexamethasone（DXM）， AS extract and AS extract+DXM， respectively. Pathological examination of lung tissues was conducted by HE staining， and ELISA was used to detect the levels of IL-4， IL-17， IFN-γ， TGF-β as well as retinoic acid receptor-related orphan receptor （RORγt）. Results showed that AS could significantly improve the situation of inflammation infiltration， increase ratios of IFN-γ/IL-4 and TGF-β/IL-17， decrease the levels of RORγt in lung tissues. The AS+DXM group showed a best treatment effect. The results indicated that AS played a therapeutic role for asthma with Yin deficiency syndrome and improved airway inflammation by inhibiting the expression of RORγt in lung tissues and regulating the balance of Th1/Th2 and Th17/Treg. 　　[Key words] Angelicae Sinensis Radix； asthma with Yin deficiency syndrome； Balb/c mouse； Th1/Th2； Th17/Treg 　　哮喘（asthma）是我??临床中常见的一种呼吸系统疾病，其临床发作具有周期性、反复性、迁延不愈的特点[1-2]。2013年哮喘流行病学调查数据显示，我国大陆地区哮喘总患病率高达1.24%，严重威胁公众健康[3]。研究表明，哮喘发病的主要病理学机制是辅助性T细胞（Th）亚群的免疫功能失调，其中，Th1/Th2与Th17/调节性T细胞（Treg）的功能平衡是影响呼吸道炎性因子分泌及促炎症反应的关键