当归红芪超滤膜提取物对过氧化氢致PC12细胞凋亡影响.docVIP

当归红芪超滤膜提取物对过氧化氢致PC12细胞凋亡影响 　　摘要：目的 观察当归红芪超滤膜提取物对过氧化氢（H2O2）致PC12细胞凋亡的影响，探讨其作用机制。方法 H2O2诱导PC12细胞凋亡，构建氧化损伤神经细胞凋亡模型。实验分为正常对照组、模型组及中药低、中、高剂量组，中药各组给予不同剂量当归红芪超滤膜提取物。流式细胞术检测细胞凋亡率，激光共聚焦显微镜检测细胞线粒体膜电位变化，Western blot检测Bax、Bcl-2和cleaved Caspase-3的蛋白表达。结果 与模型组比较，中药各剂量组细胞凋亡率明显降低，线粒体膜电位升高，Bax、cleaved Caspase-3蛋白表达均减弱，Bcl-2表达增强，Bcl-2/Bax比值升高（P0.05）。结论 当归红芪超滤膜提取物具有抗H2O2诱导的PC12细胞凋亡的作用。 　　关键词：当归红芪超滤膜提取物；过氧化氢；PC12细胞；凋亡 　　DOI：10.3969/j.issn.1005-5304.2016.06.018 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2016）06-0068-05 　　Abstract： Objective To observe the effects of ultrafiltration extract from Angelica Sinensis Radix and Hedysari Radix on PC12 cell apoptosis induced by H2O2； To discuss its mechanism of action. Methods H2O2 was used in the incubation of PC12 cells to establish the oxidative damage nerve cell apoptosis model. The experiment was divided into normal control group， model group， and three different dosages （0.375， 0.75， 1.5 g/L） of ultra-filtration extracted from Angelica Sinensis Radix and Hedysari Radix after modeling for interference. Rate of cell apoptosis was detected by flow cytometry； mitochondrial membrane potential was measured by laser scanning confocal microscopy； the protein expressions of cleaved Caspase-3， Bax and Bcl-2 were detected by Western blot. Results Compared with the model group， rates of cell apoptosis in the different dosage groups decreased significantly； membrane potential of mitochondria increased； the protein expressions of cleaved Caspase-3 and Bax decreased； the expression of Bcl-2 increased； the ratio of Bcl-2 and Bax increased （P0.05）. Conclusion The ultra-filtration extracted from Angelica Sinensis Radix and Hedysari Radix can inhibit PC12 cell apoptosis induced by H2O2. 　　Key words： ultra-filtration extract from Angelica Sinensis Radix and Hedysari Radix； H2O2； PC12 cells； apoptosis 　　当归红芪超滤膜提取物（ultra-filtration extract from Angelica Sinensis Radix and Hedysari Radix，UFE-AH）具有抗自由基、抗氧化损伤等功效[1-4]。过氧化氢（H2O2）是活性氧族（ROS）的主要成分之一，能够损伤生物膜和线粒体，是建立