中药平消栓对h 22肝癌移植瘤模型小鼠fas和mmp 9表达影响的实验研究-effect of pingxiao suppository on fas and mmp - 9 expression in h 22 liver cancer transplanted tumor model mice.docxVIP

The Exper i ment al St udy Of The Pi ngXi ao Supposi t or y Eff ect ed The Expr essi on Of The Fas and MMP- 9 I n Li ver Cancer Mouse Abst r act Obj ect i ve: Thr ough st udy of t he t r adi t i onal Chi nese medi ci ne Pi ngPi ao supposi t or y t o H22 asci t es car ci noma of t he I CR st r ai n mi ce t r ansf ect ed wi t h t he gener al pr oj ect s, i mmune or gans ( spl een, t hymus) , Fas and MMP- 9 expr essi on of exper i ment al st udi es t o i nvest i gat e t he ant i - t umor dr ug possi bl e mechani sm f or cl i ni cal appl i cat i on and f or t he f ur t her devel opment of Chi nese medi ci ne i n cancer r esear ch and pr ovi de a t heor et i cal basi s. Met hods: Based on t he r andomi sat i on pr i nci pl e, di vi des i nt o st ochast i cal l y 40 I CR f emal e mouse f our gr oups, r espect i vel y be t he bl ank cont r ol gr oup ( A gr oup) , t he Dut ch l ump cont r ol gr oup ( B gr oup) , 5 f l uor i ne ur acil gr oup ( C gr oup) , t he Pi ng xi ao Supposi t or y gr oup ( D gr oup) . The A gr oup does not make any deal wi t h and t he B, C, D gr oup each mouse r i ght ar mpi t depar t ment hypoder mi c vacci nat es t he H22 l ump cell t o hang f l ui d 0. 2ml , aft er vacci nat i ng 24h wei ghi ng, and st ar t s t he abdomi nal cavi t y i nj ect i on t o gi ve t he medi ci ne: A, B gr oup uses physi ol ogi cal sal i ne 0. 2ml / onl y ( cont i nuousl y t o gi ve medi ci ne 14 days) ever y day, C gr oup wi t h 5 f l uor i ne ur aci l mg/ day, di l ut es t he densi t y i s mg/ 0. 2ml ( t he 1～5t h day f or medi ci ne) , D gr oup wi t h nor mal sal i ne wi l l be made i nt o 1. 0g/ 10ml l evel Supposi t or y concent r at i on i n aqueous sol ut i on ( f or consecut i ve 14 days) . Obser ves t he mouse f eed, t he pot abl e wat er , t he act i ve si t uat i on, t he st at e of mi nd, Response t o ext er nal st i mul at i on, t he super f i ci al knowl edge l ust er ever y day, and si t uat i