心力衰竭大鼠IL6变化及卡托普利干预作用.docVIP

心力衰竭大鼠IL6变化及卡托普利干预作用 　　【 摘要】 目的 了解IL-6在阿霉素诱导心力衰竭大鼠过程中的改变，以及卡托普利的干预作用。 方法 实验中选用Sprague-Dawley(SD)大鼠30只，雌雄各半，随机分为正常对照组、心力衰竭组及心力衰竭治疗组。经腹腔注射阿霉素建立心力衰竭模型，模型成功后正常对照组和心力衰竭组口服灌注安慰剂，心力衰竭治疗组口服灌注卡托普利每天2.5 mg/100 g。于口服灌药4周后处死动物检测指标。采用开胸直视下心室腔内插管测心室内压，颈总动脉插管测动脉压，压力传感器记录左室血流动力学改变，放免法测定血清IL-6的浓度，透射电镜下观察心肌病理学改变，通过全心重量指数测定脏器比。 结果 1 心力衰竭组大鼠血浆中IL-6显著升高，与正常对照组 比较（P0.05）；心力衰竭治疗组在使用卡托普利后IL-6明显下降（P0.05）。2. 心力衰竭组左室血流动力学与正常对照组相比呈现显著性差异（P0.05）；心力衰竭治疗组在使用卡托普利后左室血流动力学明显改善，与心力衰竭组相比差异有统计学意义（P0.05）。 结论IL-6在心力衰竭的发生和发展过程中起重要作用，卡托普利通过抑制IL-6的升高而减轻炎症反应，从而预防心室重构，改善心功能。 　　【关键词】IL-6；心力衰竭；卡托普利；心功能 　　Change of IL-6 in heart failure rats and effect of captopril 　　YAN Yan.The Second Department of Cardiology, the General Hospital of Fushun Mining Group, Fushun, Liaoning 113008, China 　　 　　[Abstract] ObjectiveTo evaluate the change of interleukin-6 (IL-6) in heart failure rats induced by adriamycin and effect of captopiril. Methods30 Sprague-Dawley rats were randomly divided into three groups: normal group, control group and captopril group. The model of heart failure was formed by administering adriamycin to rats. After the model succeeded, normal group and control group were treated with placebo. Captopril group was treated with captopril (2.5mg#8226;100g-1#8226;d-1). Four weeks later, hemodynamic indexes including HR, ASP, ADP, LVSP, LVEDP and ±dp/dtmax were essayed in each group. The circulation level of IL-6 was detected by RIA. The hearts were harvested to test HWI (heart weight index) and the ultrastructural features of left ventricular were observed by transmission electron microscope (TEM). Results 1. Compared with normal group , the level of IL-6 notably rose in control group (P0.05); compared with conrol group, the level of IL-6 obviously decreased in captopril group (P0.05).2. Compared with normal group , left ventricular hemodymic parameters were notably different in control group (P0.05); Compared with control group, the paremeters in captopril group were notably different (P0.05).Conclusio