转sacb和kate基因棉花抗旱性的分析-analysis of drought resistance of cotton transferred with sacb and kate genes.docxVIP

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转sacb和kate基因棉花抗旱性的分析-analysis of drought resistance of cotton transferred with sacb and kate genes.docx

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转sacb和kate基因棉花抗旱性的分析-analysis of drought resistance of cotton transferred with sacb and kate genes

II万方数据 II 万方数据 Abstract Through pollen tube channel method to import E. coli catalase gene KatE (fusion Arabidopsis chloroplast transit peptide STP) and Bacillus Subtilis fructan synthase gene SacB (fusion positioning vacuole of sweet potato transit peptide SP) cotton T3 on behalf of the test material, the kanamycin detection and PCR identification, screening positive transgenic plants. In the field ,when the transgenic and the control cotton plant grew into seedling stage, bud stage and flowering stage three key growth period, by shearing the drip irrigation belt to produce continuous drought stress treatment. Through the determination of physiological and biochemical indexes, agronomic traits of investigation to preliminary analysis of transgenic cotton in the field of drought resistance.The experimental results are as follows: Cotton drops after the emergence of water, Cut drip irrigation belt， transgenic and control cotton were proceeded continuous drought stress treatment from seedling stage. Drought stress to the seedling stage, bud stage and flowering stage, transgenic and control cotton leaf physiological and biochemical indexes were respectively determined in different growth period. The results show that, there were no significant differences on the phenotype and leaf relative water content between transgenic and control plants, but KatE cotton chlorophyll content was significantly higher than control.As the extension of drought stress time, the cotton in drought stress to the bud stage and flowering stage,the transgenic cotton leaf relative water content, chlorophyll content, proline content and SOD activity, Fv/Fm value, net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (Tr) are of different level is higher than contrast cotton, respectively reached significant difference or extremely significant level. Cell membrane ion leakage and malondialdehyde content determination results show that c