中西医结合治疗硬皮病模型小鼠的实验分析-experimental analysis of treating scleroderma model mice with integrated traditional chinese and western medicine.docxVIP

下载本文档

42
0
约4.43万字
约 51页
2018-09-10 发布于上海
举报
版权申诉

中西医结合治疗硬皮病模型小鼠的实验分析-experimental analysis of treating scleroderma model mice with integrated traditional chinese and western medicine.docx

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

中西医结合治疗硬皮病模型小鼠的实验分析-experimental analysis of treating scleroderma model mice with integrated traditional chinese and western medicine

成都中医药大学成都中医药大学 2005 级硕士学位论文 4 4 24205.81±2045.99, 11533.49±1651.55, the IOD of TGF-β were 7892.33± 255.98, 3018.29±208.66, the thickness of dermis was markedly thickened (P 0.05), the IOD of collagen fibers and TGF-β were obviously increased (P 0.05). 2. In the groups of model control, western medicine, Chinese medicine, Chinese-western medicine, the thickness of dermis(μm) were 618.33±31.25, ±34.25, 484.66±42.98, 423.22±37.26, the IOD of collagen fibers were 24746.41±2548.21, 18545.98±1683.31, 19317.37± 1553.28, 7317.02±1426.39, the collagenic quantity (μg/ml) were 6905.62±241.16, 5135.29±302.52, 5461.36±395.54, 4970.29±338.96, the level of CTGF (pg/ml) were 564.44± 56.31, 416.11±63.77, 427.78±53.33, 361.67±41.68, the IOD of TGF-β were 7996.96±286.87, 6161.27± 399.62, 6178.91±403.04, 5632.01±331.62. Compared with the model controls, the groups treated by drugs showed atendency to ameliorate the thickened dermis, the collagenic quantity, the levelof CTGF of serum and the IOD of TGF-β all markedly decreased (P 0.05).The direct correlation between the content of TGF-β and CTGF was found (P 0.05). Conclusion：The animal model of scleroderma can be successfully established in Balb/c mouse with BLM. Treatment with Chinese-western medicine had the role of ameliorating the skin and preventing the collagen fibers hyperplasy, which is better than treated by western medicine or Chinese medicine alone. The mechanism of action may be realized through these links as follows: decreased collagenic quantity and level of CTGF, inhibited the expression of TGF-β. Key words：Scleroderma; mouse model; combined treatment of traditional Chinese medicine and western medicine; Experimental study 6 6 缩略词英文缩写英文全称中文 BLM bleomycin 博莱霉素 CTGF connective tissue growth factor 结缔组织生长因子 ELISA enzyme-linked immunosorbent assay 酶联免疫吸附测定 ECM extra cellular matrix 细胞外基质 HE hematoxylin-eosin 苏木素-伊红染色 IHC Immunohistochemistry 免疫组织化学 IOD integrated optical density 积分光密度 OD optical density 光密度 PBS ph