猪流行性腹泻病毒coe抗原表位的原核表达及间接elisa检测方法的建立-prokaryotic expression of coe antigen epitope of porcine epidemic diarrhea virus and establishment of indirect elisa detection method.docxVIP

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猪流行性腹泻病毒coe抗原表位的原核表达及间接elisa检测方法的建立-prokaryotic expression of coe antigen epitope of porcine epidemic diarrhea virus and establishment of indirect elisa detection method.docx

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猪流行性腹泻病毒coe抗原表位的原核表达及间接elisa检测方法的建立-prokaryotic expression of coe antigen epitope of porcine epidemic diarrhea virus and establishment of indirect elisa detection method

PAGE PAGE IV III III Abstract Porcine epidemic diarrhea is a highly contagious intestinal infectious disease which caused by porcine epidemic diarrhea virus.All ages pigs are thought to be susceptible to infection. It occur frequently around the world and result in serious economic losses for the pig industry. Since 2010,PED occur in isolation or mixed infections with other pathogens.It cause severe diarrhea of piglets of various scale pig farms.Morbidity and mortality were significantly higher.The vaccine is an important means of prevention and treatment of PED.While the cultured in vitro of PEDV is difficult,virus content of vaccine tends to be insufficient, which can cause immune failure.In addition,the PEDV attenuated vaccine for the suckling pig exists the residual virulence. There will be the risk of pathogenic.Therefore,development of PEDV gene engineering vaccine has important significance on the prevention and treatment of PED.In addition,the PEDV diagnostic reagents used at home and abroad are mostly whole virus.But the separation and cultured in vitro of PEDV is very difficult,so a large number of antigen preparation is extremely difficult.In summary, the test PEDV strains of the S gene popular in recent years, the protective antigen (COE) of the conservative further genetic analysis with COE prokaryotic expression protein PEDV indirect ELISA diagnostic techniquesand to provide for the the PEDV new genetically engineered vaccine theory and material basis.This test analysised the conservative of the protective antigen gene (COE) of S gene of PEDV strains which is popular in recent years,established indirect ELISA diagnostic techniques of PEDV with COE prokaryotic expression protein,and provide theoretical and material basis of the new genetic engineering preparation vaccine.Studies are as follows: This test designed one pair of primers P1,P2 according to PEDV CV777 strain complete gene sequence (accession number as AF353511) from GenBank,and amplified C