重叠细胞的判别与分割计数方法分析-identification of overlapping cells and analysis of segmentation and counting methods.docxVIP

III III 7 ~//太原理工大学硕士 7 ~/ / RESEARCH ON OVERLAPPING CELL IDENTIFICATION AND SEGMENTATION COUNTING METHOD ABSTRACT In biomedical image，applied computer assistive technology on medical image processing has become main tools for cl inical diagnosis and pathological analysis. ln cell image owning to their own structure ，multiple cells 0丘en overIap，which for cell image segmentation and the count is an important basis to observe and analyze whether there are disease. Cell image acquisition needs hardware，softwal飞 and many other aspects of work to do，so how to use the applied image processing and analysis technology to cut up over1apping cells image in the complex background ，used to improve the accuracy of medical analysis and work efficiency for medical workers is the focus ofthesis. The key factor distinguishing whether or not cells overlapped is the shape factor in morphological parameter which is an effective parameter in a measure of cell close roundness，wben cells overlap ，their boundaries due to sag wil1 coηespondingly lead to sbape factor decrescent ，therefore，we can use shape factor to effectively identif旨 whether ceIls overlap to prepare for subsequent segmentation and count. In this paper，由e method of calculation of cell perimeter has been improved to remove the tiny burrs on 由e edge of cel1s and the noise，rnaking it more coηespond with rounded outline of cells and proposed regional pixel labeling method based on boundary peeling-off ，which avoids the problems that the cells in the process of tracking into dead circulation ，and shortens the running time of the algorithm，simplifies tbe design procedure. Cell image due to differences in the collection process causes differences and unsatisfactory degradation of image quaJity. 10 order to more accurately JV JV 太原理工大学硕士研究 生学位论文 show the outlines of cells，we remove the internaJ hoJe in image，and fill the image and lhen lhe image looks much smoother. In the appJication of mathematical mo甲hoJogy the grad