抑肽酶在肠IR中对血清PLA2影响.docVIP

抑肽酶在肠IR中对血清PLA2影响 　　[摘要] 目的：应用肠系膜上动脉阻断模型，探讨肠I/R损伤对血清PLA2的影响，以及抑肽酶的肠保护作用机制。从而为抑肽酶在临床中的应用提供一定的理论依据。方法：24只日本大耳白兔随分组，空白对照组A组SMA不作阻断，B组为缺血/再灌注组，C组为抑肽酶治疗组，B、C两组SMA均作阻断。采用家兔肠系膜上动脉阻断（SMAO）模型，阻断时间为45min。C组阻断前5min静注抑肽酶30000KIU?Kg-1，随后以10000KIU?Kg-1?h-1维持至实验结束。B、C组在阻断前（Io）、阻断45min（I1），再灌注1h（R1）、再灌注2h（R2）各时点观察血清MDA、PLA2及肝脏组织形态学变化。A组在相应时点取样检测上述指标。结果：（1）C组PLA2在R2时血清值为：32.60±3.075μ，明显低于B组相应值：86.725±13.38μ（P0.05）。而B组明显高于A组值（P0.05）。结论：抑肽酶不仅减轻脂质过氧化反应，对肠缺血再灌注损伤有一定保护作用，而且还可以减少PLA2，减轻肠I/R过程中对器官的损害，对肠道亦可起到一定的保护作用。 　　[关键词] 抑肽酶；缺血/再灌注损伤；磷脂酶A2（PLA2） 　　[Abstract] Objective:Study the effect of the intestinal ischemia reperfusion injury on the liver and another organ using SMAO model,To observe the protective effect of aprotinin on PLA2 during the intestinal ischemia reperfusion injury.Materials and Method:24 rabbits were randomly divided into three groups (n=8):Control group (A),SMA wasnot obstructed.ischemia/reperfusion group(B) and aprotiin group (C). SMAO model was prepared and obstructed time was 45min , and reperfusion for 2h. In the group C , aprotinin was injected into Vein 5min before clamping at 30000KIU?Kg-1, and was kept on at 10000 KIU?Kg-1?h-1,malondialdehyde (MDA),Phospholipase A2( PLA2)of serum were measured before obstruction ( I 0), at claming for 45min (I1)and 1h( R1), 2h(R2) after reperfusion respectively.The livet was examined for morphological changes.Results: PLA2 of serum at R2 in the group C were 32.6+-3.075u, were significantly lower than those of group A(P0.05).But the level of group B were markly higher than those of group A,(P0.05).Conclusion:Aprotinin have protextive dffdcts on the intestinal ischemia-reperfusion,inhibt the level of lipid peroxidation, and declin the level of PLA2 in the serum the damage on another organ in the intestinal ischemia-reperfusion. 　　[Key words] Aprotinin;ischemia/reperfusion of intestinal ;Phospholipase A2 　　应用肠系膜上动脉阻断模型，探讨肠I/R损伤对血清 PLA2的影响，以及抑肽酶的肠保护作用机制。从而为抑肽酶在临床中的应用提供一定的理论依据。 　　实验证明，它对肠缺血再灌注损伤的一定的保护作用，抑肽酶是否可以降低 PLA2的血清水平，我们作如