补肾活血针刺法对SAMP8小鼠杏仁核蛋白质组学表达的影响.docVIP

补肾活血针刺法对SAMP8小鼠杏仁核蛋白质组学表达的影响 　　摘要：目的 观察补肾活血针刺法对阿尔茨海默病（AD）模型小鼠SAMP8杏仁核蛋白质组学表达的影响，探寻针刺治疗AD的潜在靶点蛋白。方法 30只6月龄雄性SAMP8小鼠随机分为针刺组和对照组，每组15只，针刺组选取“肾俞”“百会”“血海”“膈俞”进行干预，对照组予以相同时间捉抓刺激。干预8周后，取杏仁核，采用蛋白质组学技术鉴定差异蛋白质点。结果 与对照组比较，针刺组最终鉴定有9个差异蛋白质点，其中6个上调、3个下调，根据蛋白质数据库中提供的相关信息，差异蛋白质点功能主要涉及线粒体能量代谢、氧化应激、β-淀粉样蛋白（Aβ）产生等方面。结论 补肾活血针刺法可调节杏仁核内多种蛋白质的表达，提示其可能是通过改善线粒体能量代谢、抗氧化应激、减少Aβ产生等来实现对AD的潜在治疗作用。 　　关键词：阿尔茨海默病；补肾活血；针刺；SAMP8小鼠；杏仁核；蛋白质组学 　　DOI：10.3969/j.issn.1005-5304.2018.03.013 　　中图分类号：R245 文献标识码：A 文章编号：1005-5304（2018）03-0058-06 　　Abstract： Objective To observe the effects of Bushen Huoxue acupuncture method on amygdaloid protein expression in SAMP8； To explore the potential target protein for acupuncture treatment of Alzheimer disease （AD）. Methods Thirty six-month-old male SAMP8 mice were randomly divided into acupuncture group and control group， 15 mice in each group. Acupuncture group selected Baihui （GV20）， Shenshu （BL23）， Geshu （BL17） and Xuehai （SP10） to intervene. The control group was given the same time to catch and stimulate. After 8 weeks， the amygdala was extracted and the differential expression protein spots were identified by proteomic techniques. Results Compared with control group， acupuncture group eventually identified 9 differential expression protein spots， of which 6 up-regulated and 3 down-regulated. According to the relevant information provided in the protein database， the main function of differential expression proteins involved in the mitochondrial energy metabolism， oxidative stress， and production of Aβ. Conclusion Bushen Huoxue acupuncture method can regulate multiple protein expressions in amygdala， suggesting that it may be through improving mitochondrial energy metabolism， oxidative stress， reducing production of Aβ to realize the potential therapeutic effects on AD. 　　Keywords： Alzheimer disease； Bushen Huoxue； acupuncture method； SAMP8 mice； amygdala； proteomics 　　阿尔茨海默病（Alzheimer disease，AD）是最常?的痴呆类型之一，也是最常见的神经变性疾病，以记忆力减退、认知功能障碍及人格改变为主要临床表现，其病理特征是β-淀