散斑竹根七组织培养与快速繁殖体系建立.docVIP

散斑竹根七组织培养与快速繁殖体系建立 　　摘要：以散斑竹根七[Disporopsis aspera （Hua） Engl.ex Krause]的嫩茎段作为外植体，研究了其组织培养和快速繁殖体系。结果表明，外植体预处理后，经过75%的乙醇消毒20 s，再用0.1% HgCl2处理6 min的灭菌效果最好，启动率达到78.40%，污染率仅为23.75%。初代培养最适宜的培养基是MS+ 6-BA 1.0 mg/L+2，4-D 0.01 mg/L+NAA 0.1 mg/L+IAA 1.0 mg/L；MS+ 6-BA 0.5 mg/L+KT 1.5 mg/L +NAA 0.2 mg/L+IBA 1.0 mg/L适用于继代增殖培养，丛生芽的增殖系数达到6.10；适宜生根的培养基为NAA 1.0 mg/L+IBA 0.5 mg/L+1/2MS，生根率达到96.13%。 　　关键词：散斑竹根七[Disporopsis aspera （Hua） Engl.ex Krause]；组织培养；快速繁殖体系 　　中图分类号：Q943.1 文献标识码：A 文章编号：0439-8114（2015）16-4070-03 　　DOI：10.14088/j.cnki.issn0439-8114.2015.16.062 　　Establishment of Rapid Propagation System for Disporopsis aspera （Hua） Engl.ex Krause by Tissue Culture 　　JIANG Tian-Yi1，TANG Min1，YANG Shu1，WANG Bo2，SHI Da-xing3 　　（1.Department of Agriculture and Horticulture，Chengdu Vocational College of Agricultural Science and Technology，Chengdu 611130， China； 2.Construction Engineering Department of Sichuan Engineering Technical College，Deyang 618000，Sichuan， China； 　　3.College of Forest Science， Sichuan Agricultural University，Ya’an 625014， Sichuan， China） 　　Abstract： Tissue culture and rapid propagation system of Disporopsis aspera （Hua） Engl.ex Krause were studied by using the tender stem as explants.The results showed that after 75% alcohol disinfection for 20 s ，with another 0.1% HgCL2 for 6 min was the best disinfection method， the survival rate was 78.40% and the contamination rate was only 23.75%. The optimum initial medium was MS+ 6-BA 1.0 mg/L+2，4-D 0.01 mg/L+NAA 0.1 g/L+IAA 1.0 mg/L； MS+ 6-BA 0.5 mg/L+KT 1.5 mg/L+NAA 0.2 mg/L+IBA 1.0 mg/L was the suitable medium for buds multiplication，tufted bud proliferation coefficient reached 6.10. The suitable rooting culture medium was NAA 1.0 mg/L+IBA 0.5 mg/L+1/2MS，which gave a rooting rate as high as 96.13%. 　　Key words： Disporopsis aspera （Hua） Engl.ex Krause；tissue culture；rapid propagarion system 　　散斑竹根七[Disporopsis aspera （Hua） Engl.ex Krause]是百合科竹根七属植物，为多年生草本[1]，其叶厚纸质，花黄绿色，具紫色斑点，花被钟状[2]，春季开花，花朵俯垂，枝形优美。该种原产于林下、阴蔽山谷或溪