散结通络方对缺血性肾病晚期大鼠MMP―2TIMP―2表达影响.docVIP

散结通络方对缺血性肾病晚期大鼠MMP―2TIMP―2表达影响 　　[摘要] 目的 探?散结通络方对缺血性肾病晚期大鼠肾组织基质金属蛋白酶2（MMP-2）、基质金属蛋白酶组织抑制因子2（TIMP-2）表达的影响。 方法 将SD雄性大鼠按随机数字表法分为假手术组、模型组（均给予生理盐水3 mL/d）、药物组[散结通络方10.85 g/（kg?d）]，采用单侧肾动脉不全结扎术建立模型。于实验第28天检测大鼠24 h尿蛋白定量及血肌酐。处死大鼠，取肾组织做光镜检查，观察各组大鼠肾脏形态学改变；免疫组化法检测肾组织MMP-2、TIMP-2表达。 结果 光镜下模型组和药物组大鼠的肾小球及肾小管间质均有损伤。与假手术组比较，模型组和药物组肾小球硬化指数评分及肾小管间质损伤评分均明显升高（P 0.01），而药物组较模型组降低（P 0.01）。与模型组比较，药物组MMP-2表达升高（P 0.01），24 h尿蛋白定量、血肌酐及TIMP-2表达降低（P 0.01），且两组均高于假手术组（P 0.01）。 结论 散结通络方可能通过上调MMP-2、下调TIMP-2在肾组织中的表达对缺血性肾病晚期病变起防治作用。 　　[关键词] 散结通络方；缺血性肾病；基质金属蛋白酶2；基质金属蛋白酶组织抑制因子2；大鼠 　　[中图分类号] R692 [文献标识码] A [文章编号] 1673-7210（2017）10（b）-0024-04 　　Effects of Sanjie Tongluo Decoction on the expression of matrix metalloproteinase 2， tissue inhibitor of metalloproteinase 2 in rats with ischemic nephropathy of advanced stage 　　WANG Rui1 CHEN Guimei1 LI Wei1 LIU Lanying1▲ LI Jiaoyan1 LI Genmao2 GE Dongyu2 WANG Yaoxian3 　　1.Department of Nephrology， the Third Affiliated Hospital of Beijing University of Chinese Medicine， Beijing 100029， China； 2.Basic Research Center， Beijing University of Chinese Medicine， Beijing 100029， China； 3.Department of Nephrology， Dongzhimen Hospital， Beijing University of Chinese Medicine， Beijing 100700， China 　　[Abstract] Objective To investigate the effects of Sanjie Tongluo Decoction on the expression of matrix metalloproteinase 2 （MMP-2）， tssue inhibitor of metalloproteinase-2 （TIMP-2） of nephridial tissue in rats with ischemic nephropathy of advanced stage. Methods Male SD rats were randomly divided into sham operation group， model group （both groups were given normal saline 3 mL/d）， drug group [Sanjie Tongluo Decoction 10.85 g/（kg?d）] by random number table method. The unilateral renal artery insufficiency ligation was used to establish animal model. In the 28th day of experiment， the levels of 24 hours urinary protein and creatinine of rats were detected. The rats were put to death and nephridial tissue was collected to take light microscope examination， the changes