新伐他仃对单肾切除糖尿病老龄鼠肾脏波形蛋白表达影响.docVIP

新伐他仃对单肾切除糖尿病老龄鼠肾脏波形蛋白表达影响 　　【摘要】目的:观察辛伐他仃对单肾切除糖尿病老龄鼠血生化指标、波形蛋白表达,探讨新伐他仃的肾脏保护作用。方法:32只15月龄清洁级SD大鼠随机分为正常对照,肾切对照,糖尿病组,新伐他仃组。给予相应治疗后12周测定大鼠生化指标,对肾组织HE、PASM染色,镜下观察组织,免疫组化检测波形蛋白表达。结果:糖尿病组生化指标较对照组显著升高?P0.05?;肾小球系膜增厚,足突融合,基底膜增厚?P0.05?;肾小管扩张,上皮细胞水肿,损伤指数显著升高?P0.05?;治疗组尿素氮、肌酐等较糖尿病组显著下降?P0.01);治疗组甘油三酯、胆固醇与糖尿病组相比无明显变化?P?0.05?;治疗组与糖尿病组相比波形蛋白表达显著下降?P0.01?。结论:治疗后肾脏病理改善,免疫组化发现新伐他仃减少波形蛋白异常表达。 　　【关键词】新伐他仃;老龄鼠;波形蛋白;单肾切除 　　 　　doi:10.3969/j.issn.1006-1959.2010.05.019 文章编号:1006-1959(2010)-05-1047-03 　　Protective effect of simvastatin in uninephrectomize STZ induced diabetic aging rat Master candidate LiuxuequnLIU Xuequn,SHI Hui 　　【Abstract】Objective:To explore the protective effect of simvastatin on the kidney of uninephrectomized STZ induced diabetic aging rat.Method:Thirty two fifteen-month-old SD rats were randomly assigned to normal control group,uninephrectomized control group,simvastatin treatment group and Diabetic group After 12 weeks,biomedical markers were determined,the renal pathology changes were observed under light and electron;expression of vimentin were detected by immunohistochemistry.Results:biomedical markers of group D were singnifcantly increased compared with those of group C?P0.05?,markers of group S were decreased compared with those of group D?P0.05?;mean glomerular area and total number glomerular cells of group D were higher than those of other groups ?P0.05?,expression of vimentin in group S were decreased compared with those in group D.Conclusion:treatment with simvastatin,the pathology of kidney were improved;simvastatin may play a protective role by downregulating vimentin. 　　【Key words】Uninephrectectomize;Aging;Simvastatin;Vimentin 　　 　　他仃类是一种临床常用的降血脂药物,通过对胆固醇合成途径的限速酶HMG-COA还原酶的抑制,降低血清蛋固醇,最近肾脏疾病的临床和实验研究中发现,他汀类药物的肾保护作用并不完全依赖降脂效应,中间纤维具有严格的组织特异性,来源于同一基因家族,具有高度同源性,中间纤维与细胞分化关系密切,波形蛋白是一种中间纤维蛋白,由单一多肽链构成,主要分布在中胚层来源的组织中,而角蛋白质纤维存在于上皮细胞,结蛋白纤维,存在于肌细胞。研究发现肾脏固有细胞转分化在肾脏纤维化发生发展中起十分重要作用。其转化程度与肾功能下降的程度相平行。通过老龄鼠单肾切除后,给予一次性腹腔注射40mg/kg链脲佐菌素,破坏胰岛β细胞,制成糖尿病模型,辛伐他汀(8mg/k