止嗽散加减方对慢性阻塞性肺疾病寒燥模型大鼠表皮生长因子受体及中性粒细胞弹性蛋白酶影响.docVIP

止嗽散加减方对慢性阻塞性肺疾病寒燥模型大鼠表皮生长因子受体及中性粒细胞弹性蛋白酶影响 　　摘要：目的 ?^察止嗽散加减方对慢性阻塞性肺疾病（COPD）寒燥模型大鼠表皮生长因子受体（EGFR）及中性粒细胞弹性蛋白酶（NE）的影响。方法 利用气管滴注弹性蛋白酶结合烟熏90 d建立COPD模型，在此基础上予以寒燥应激90 d建立COPD寒燥模型。将COPD寒燥模型大鼠随机分为寒燥组和中药组，中药组连续干预7 d。第97日处死大鼠，观察其肺组织病理形态。RT-qPCR和Western blot分别检测肺组织EGFR mRNA和蛋白表达，ELISA检测血清和肺泡灌洗液NE含量。结果 COPD组、寒燥组、中药组肺组织EGFR mRNA和蛋白表达较对照组均升高，中药组肺组织EGFR蛋白表达较COPD组和寒燥组均降低；COPD组、寒燥组和中药组血清和肺泡灌洗液NE含量较对照组升高，寒燥组肺泡灌洗液NE含量高于COPD组，中药组肺泡灌洗液NE含量低于寒燥组。结论 止嗽散加减方可能通过降低寒燥COPD模型大鼠肺组织EGFR和NE的水平，达到治疗COPD的目的。 　　关键词：慢性阻塞性肺疾病；寒燥；止嗽散加减方；表皮生长因子受体；中性粒细胞弹性蛋白酶 　　DOI：10.3969/j.issn.1005-5304.2017.10.013 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2017）10-0053-04 　　Effects of Modified Zhisou Power on Epidermal Growth Factor Receptor and Neutrophil Elastase in Rats with Cold-Dryness Chronic Obstructive Pulmonary Disease GAO Zhen， WANG Jing， JIANG Min， LI Zheng， XU Dan， Halmurat UPUR， JING Jing， LI Feng-sen （National Clinical Research Base of Traditional Chinese Medicine， Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University， Urumqi 830000， China） 　　Abstract： Objective To reveal the effects of cold-dryness and modified Zhisou Power on epidermal growth factor receptor （EGFR） and neutrophil elastase （NE） in rats with COPD. Methods COPD model was established with an elastase dose into the trachea combined with exposure to smoking for 90 d； cold-dryness COPD group was further developed by exposure to a cold， dry environment for 90 d. After 90 days， cold-dryness COPD rats was divided into cold-dryness group and Zhisou Power intervention group （treated with modified Zhisou Power for 7 days）. On the 97th day， all rats were killed. Pathological changes in lungs were observed. mRNA and protein expression of EGFR were measured by RT-qPCR and Western blot， and the amount of NE in serum and BALF were examined by ELISA. Results EGFR mRNA and protein expression were significantly higher in COPD group， cold-dryness group and Zhisou Power intervention group than in control group. EGFR was significantly