淋巴细胞亚群在反复喘息婴幼儿血清中表达水平研究.docVIP

淋巴细胞亚群在反复喘息婴幼儿血清中表达水平研究 　　[摘要] 目的 研究反?痛?息婴幼儿血清中淋巴细胞亚群的变化和意义。 方法 方便收集南京医科大学第二附属医院2013年6月―2015年1月住院反复喘息患儿54例，年龄均在3岁以下，按哮喘预测指数分3组，预测指数阳性组（A组）、哮喘预测指数阴性组（B组）、正常对照组（C组）。哮喘预测指数阳性组22例，哮喘预测指数阴性组22例，正常对照组10例。所有患儿入院当日采集静脉血，流式细胞仪检测患儿血清T淋巴细胞（CD3+）、B淋巴细胞（CD19+），T辅助性淋巴细胞（CD3+、CD4+），T抑制性淋巴细胞（CD3+、CD8+）的水平。 结果 A组共22例，淋巴细胞免疫分型异常者17例，占总数的77.27%；B组共22例，淋巴细胞免疫分型异常者3例，占总数的13.63%；C组共10例，淋巴细胞免疫分型异常者1例，占总数的11.11%。A组与C组差异有统计学意义（P0.05）；B组与C组相比差异无统计学意义（P0.05）。 结论 反复喘息婴幼儿，哮喘预测指数阳性组淋巴细胞亚群功能失衡，而哮喘预测指数阴性组淋巴细胞亚群功能正常。 　　[关键词] 淋巴细胞亚群；喘息；婴幼儿；哮喘预测指数 　　[中图分类号] R725 [文献标识码] A [文章编号] 1674-0742（2016）12（b）-0032-04 　　Study on the Expression of Lymphocyte Subsets in the Serum of Infants and Young Children with Recurrent Wheezing 　　WANG Jin-Ya，ZHU Liu-Zhen， LU Lu，GE Lei，DING Ling 　　Second Affiliated Hospital of Pediatrics of Ningjing Medical University ， Nanjing， Jiangsu Province， 210003 China 　　[Abstract] Objective To study the changes and significance of lymphocyte subsets in the serum of infants and young children with repeated wheezing. Methods Convenient collect 54 cases of hospitalized children with recurrent wheezing in the Second Affiliated Hospital of Nanjing Medical University from June 2013 to January 2015. The tests were given to three groups according to asthma Predictive Index， which were prediction index positive group （ group A）， asthma prediction index negative group （group B）， normal control group （group C）. there were 22 cases in group A； there were 22 cases in B group，also； there were 10 cases in group C. Venous blood was collected on the day of admission for all test patients. Flow cytometry was used to detect the levels of serum T lymphocytes （CD3+）， B lymphocytes （CD19+）， T helper lymphocytes （CD3+， CD4+） and T inhibitory lymphocytes（CD3+， CD8+） in test children. Results A group of 22 cases， 17 cases of abnormal lymphocyte immune type， accounting for 77.27% of the total， the B group； 22 cases， 3 cases of abnormal lymphocyte type， accounting for 13.63% of total number of 13，63%； C