猪脑死亡对血清ET―1及TNF―αIL―1βIL―6表达影响初步研究.docVIP

猪脑死亡对血清ET―1及TNF―αIL―1βIL―6表达影响初步研究 　　【摘要】 目的：探讨“渐进式”颅内加压法诱导猪脑死亡对血清中ET-1、TNF-α、IL-1β及IL-6表达的影响。方法：雄性长白猪12头，随机分为两组，对照组：颅内置入颅内压探头及水囊导管，但不进行颅内加压；脑死亡组：颅内置入颅内压探头及水囊导管，根据颅内压和平均动脉压的关系，调整加压强度建立脑死亡模型，并通过呼吸、循环维持脑死亡状态12 h，并检测血清ET-1、TNF-α、IL-1β及IL-6水平。结果：脑死亡组ET-1水平从脑死亡0 h即逐渐升高，TNF-α、IL-1β、IL-6从脑死亡后6 h开始升高，上述因子水平与对照组比较，差异均有统计学意义（P0.05）。结论：脑死亡时TNF-α、IL-1β、IL-6等炎症因子升高相对迟缓，而ET-1水平发生变化较早，灵敏度高，这可能与其作为前炎症介质有关。 　　【关键词】 脑死亡； 炎症因子； 内皮素-1 　　Preliminary Study of Effect of Brain Death on Serum ET-1，TNF-α，IL-1β and IL-6 Expression in Pigs/ZHENG Peng-bin，WAN Chen-guang，FENG Xue-quan，et al.//Medical Innovation of China，2016，13（35）：006-010 　　【Abstract】 Objective：To investigate the influence of brain dead induced by “progressive” intracranial compression on the expression of ET-1，TNF-α，IL-1βand IL-6 in serum.Method：Twelve male Landraces were randomly assigned into two groups，the control group：intracranial pressure probe and a fogarty catheter were placed intracranially，but without compression.And the brain-dead group：intracranial pressure probe and a fogarty catheter were placed intracranially，according to the relationship between intracranial pressure and mean arterial pressure，adjust the intensity of intracranial compression to establish the brain death model，and through the respiration and circulation to maintain brain death status 12 h.The serum levels of ET-1，TNF-α，IL-1β，IL-6 were detected by radioimmunological analyzer.Result：The serum level of ET-1 in the brain death group was gradually increased from the beginning of brain death，TNF-α，IL-1β，IL-6 increased from 6 h after brain death，and the above factors were significantly different compared with those in the control group（P0.05）.Conclusion：The results of this experiment show that in brain death TNF-α，IL-1β，IL-6 and other cytokines increased relatively slow，but ET-1 levels changes earlier with high sensitivity，which may be related to as proinflammatory mediators. 　　【Key words】 Brain death； Inflammatory factor； ET-1 　　First-author’s