生脉饮复方制剂对病毒性心肌炎细胞凋亡作用研究.docVIP

生脉饮复方制剂对病毒性心肌炎细胞凋亡作用研究 　　doi：10.3969/j.issn.1007-614x.2014.22.3 　　摘要目的：研究生脉饮对病毒性心肌炎心肌细胞凋亡作用的影响。方法：用柯萨奇B病毒建立感染的心肌细胞模型，在感染后24小时将细胞分为正常对照组、病理模型组、生脉饮组和辅酶Q10组。4组分别在不同时间点观察细胞形态，并采用TUNEL法进行细胞凋亡的检测。结果：在48小时点开始检测，正常对照组无细胞凋亡，生脉饮组及辅酶Q10组均有凋亡，生脉饮组凋亡率低于辅酶Q10组（P0.05），病理模型组随着时间的推移，凋亡率增加，72小时点明显高于48小时点（P0.01），与药物干预组比较差异具有统计学意义（P0.05）。结论：生脉饮能够有效抑制病毒性心肌炎心肌细胞的凋亡，且应该早期干预。 　　关键词生脉饮；病毒性心肌炎；细胞凋亡 　　Study on the apoptosis effect of pulse-activating decoction compound preparation in viral myocarditis 　　Xiong Lihui1，Sun Dashan2 　　Basic Medical Colleg，Changchun Traditional Chinese Medicine University，1301171 　　2012 Grade Traditional Chinese medicine Professional Experimental Class，Guangzhou Traditional Chinese Medicine University（Guangzhou City，Guangdong），1301172 　　AbstractObjective：To study the effect of pulse-activating decoction compound preparation in myocardial apoptosis effect of viral myocarditis.Methods：Using coxsackie B virus established infectious myocardial cell model.In 24 hours after infection，the cells were divided into four groups：the normal control group，the pathological model group，the pulse-activating decoction group and the coenzyme Q10 group.The cellular morphology of four groups of cells were observed at different time points.The apoptosis was detected by TUNEL method.Results：Begin testing at 48 hours，the normal control group had no apoptosis；the pulse-activating decoction group and the coenzyme Q10 group all had apoptosis；the apoptosis rate of the pulse-activating decoction group was lower than that of the coenzyme Q10 group（P0.05）.In the pathological model group，the apoptosis rate was increased as time goes on.72 hours point was obviously higher than 48 hours point（P0.01）.There had statistically significant compared with the drug intervention group （P0.05）.Conclusion：Pulse-activating decoction can effectively inhibit the myocardial cell apoptosis of viral myocarditis，and it should be early intervented. 　　Key wordsPulse-activating decoction；Viral myocarditis；Apo