砂炒对波棱瓜子有效成分致腹泻以及保肝作用影响.docVIP

砂炒对波棱瓜子有效成分致腹泻以及保肝作用影响 　　[摘要]为了研究砂炒对波棱瓜子有效成分、致腹泻以及保肝作用的影响，该实验采用HPLC分析波棱瓜子砂炒前后有效成分波棱甲素与波棱素的含量差异;以稀便率和腹泻指数评价砂炒对波棱瓜子总木脂素（total lignans， TL）致泻情况的影响;以CCl4急性肝损伤模型评价砂炒对波棱瓜子TL保肝作用的影响。HPLC分析结果显示，波棱瓜子砂炒后波棱甲素含量降低40.9%（Plt;0.01），波棱素含量无显著性变化。药效学结果显示，砂炒波棱瓜子TL高、中剂量组稀便率与腹泻指数显著低于同剂量生品波棱瓜子TL组（Plt;0.01或Plt;0.05）;砂炒及生品波棱瓜子TL高、中剂量组均能显著抑制CCl4致肝损伤小鼠血清中ALT，AST水平的升高（Plt;0.01或Plt;0.05），但砂炒波棱瓜子TL高、中剂量组小鼠ALT，AST水平高于同剂量的生品波棱瓜子TL组（Plt;0.05）。结果表明，砂炒可有效缓解波棱瓜子TL致泻作用，但是同时也降低了波棱瓜子TL的保肝作用。 　　[关键词]波棱瓜子;总木脂素;砂炒;腹泻;保肝作用 　　Influence of stir-baked with sand on active ingredients， diarrhea and 　　hepatoprotection of Herpetospermum caudigerum 　　LI Juan-juan1，2， SHEN Gang1，2， YIN Rong-lilt;supgt;1lt;/supgt;， SHEN Cheng-ying1，2， CHENG Ling1，2， QIU Ling1，2， HAN Jin2*， YUAN Hai-long2* 　　（1. College of Pharmacy， Chengdu University of Traditional Chinese Medicine， Chengdu 611137， China; 　　2. 302 Military Hospital of China， Beijing 100039， China） 　　[Abstract]To study the influence of stir-baked with sand on active ingredients， diarrhea and hepatoprotection of Herpetospermum caudigerum， the contents of herperione and herpetin in H. caudigerum before and after stir-baking with sand were analyzed by HPLC. The effect of stir-baked with sand on diarrhea of H. caudigerum TL was evaluated using the mean stool rate （MSR） and mean diarrheal index （MDI） and the influence of stir-baked with sand on hepatoprotective effect of H. caudigerum TL was examined using a mouse model of CCl4-induced liver injury based on the analysis of serum ALT and AST activities. The results of HPLC analysis showed the content of herperione in H. caudigerum after stir-baking with sand decreased by 40.9% （Plt;0.01） and the content of herpetin had no change. Pharmacodynamic results showed that the MSR and MDI of high-dose and middle-dose group of H. caudigerum TL after stir-baking with sand were significantly lower than that of high-dose and middle-dose group of H. caudigerum TL without stir-baking with sand; The high-dose and middle-dose of H