种植密度与钾肥互作对烤烟成熟期多酚氧化酶及色素影响.docVIP

种植密度与钾肥互作对烤烟成熟期多酚氧化酶及色素影响 　　摘要 ?榱烁纳魄?北山地烤烟的烘烤特性，采用以种植密度为主区、钾肥施用量为副区的裂区双因素试验，结合偏（Eta）2值分析法，研究种植密度与钾肥施用量及其互作对烤烟多酚氧化酶活性（PPO）、色素含量及其比值的影响。结果表明，下部叶PPO、色素含量及其比值随种植密度的增加呈显著或极显著提高，上部叶色素的变化与之相反，中部叶则先升后降。种植密度对烤烟的PPO、色素含量及其比值都有高度影响。钾肥施用量对下部叶PPO和中部叶的类胡萝卜素含量影响度较低，对中部叶PPO有中度影响，对上部叶PPO、色素含量及其比值有高度影响；1∶3的氮钾比有利于降低中上部叶PPO，提高色素含量及其比值。种植密度与钾肥互作效应对中下部叶的PPO影响度低，对上部叶的PPO有高度影响，对色素含量及其比值均有高度影响；通过对不同部位生理指标的综合评价，以种植密度19 500株/hm2、钾肥用量303.750 kg/hm2组合最有利于改善烟叶烘烤特性。 　　关键词 烤烟；种植密度；钾肥；多酚氧化酶；色素 　　中图分类号 S572 文献标识码 A 文章编号 1007-5739（2017）17-0019-04 　　Effects of Interaction Between Planting Density and Potassium Fertilizer on Polyphenol Oxidase and Pigment in Flue-cured Tobacco at Maturity Stage 　　HAN Meng-cai 1 ZHU Ying-hua 2 * XU Zeng-han 3 WANG Mao-xian 1 ZHOU Wei-hua 1 SU Xiang-yun 4 　　WANG Ding-qiang 1 GOU Jun 1 ZHANG Xiang 1 ZENG Li 1 　　（1 Tongzi Branch of Zunyi Tobacco Company in Guizhou Province，Tongzi Guizhou 563000； 2 College of Agronomy，Anhui Agricultural University； 　　3 Research Center of Tobacco and Health，University of Science and Technology of China； 4 China Tobacco Hunan Industrial Co.，Ltd.） 　　Abstract In order to improve the curing characteristics of flue-cured tobacco in northern Guizhou，two-factor split plot design by planting density for the main area and potassium fertilizer dosage for the deputy area was conducted，at the same time combined with partial（Eta）2 value analysis，to study the effect of planting density，fertilizer dosage，and their interaction on polyphenol oxidase（PPO），pigment content，and ratio of chlorophyll to carotenoids of flue-cured tobacco. The results showed that with the increase of planting density，the PPO，pigment content and ratio of chlorophyll to carotenoids were increased significantly in the lower leaves while that of upper leaves was decreased significantly，and that of the cutters was first increase and then decrease. From the partial（Eta）2 value analysis，the planting density had a high impact on PPO，pigment content and ratio of chlorophyll to caro