稳定小鼠无精子症动物模型构建分析.docVIP

稳定小鼠无精子症动物模型构建分析 　　[摘要] 目的 分析稳定的小鼠无精子症动物模型构建的方法。 方法 选取60只清洁级C5BL/6小鼠，将小鼠按照编号，随机分为两组，化疗组和激素组各30只。分别观察药物注射后，小鼠睾丸生精小管内精子和精子细胞消失情况。 结果 化疗组小鼠在接受药物注射后第4周，睾丸生精小管内精子及精子细胞消失，化疗后20周仍未产生精子，激素组在经过苯甲酸雌二醇连续注射后，于第4周睾丸生精小管内精子以及精子细胞完全消失，连续注射第20周时，部分小鼠睾丸生精小管内发现了少量的精子细胞与精子存在。两组小鼠睾丸重量存在显著差异性，具备统计学意义（P＜0.05）。结论 激素组采用苯甲酸雌二醇注射构建的小鼠无精子症动物模型缺乏稳定性，化疗组经过白消安、环磷酰胺注射后构建的小鼠无精子症模型较为稳定，是构建小鼠无精子症动物模型的首选方法，值得进行推广应用。 　　[关键词] 小鼠；无精子症；动物模型；效果 　　[中图分类号] R332#8195;#8195;#8195;[文献标识码] A#8195;#8195;#8195;[文章编号] 2095-0616（2014）11-30-03 　　 　　Analysis of stable mice azoospermatism animal model building 　　MA#8195;Zhi#8195;#8195;MENG#8195;Jiangping 　　Assisted Reproduction Center,First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China 　　[Abstract] Objective To analyze the methods of stable mice azoospermatism animal model building. MethodsSixty mice with clean grade C5BL/6 were randomly divided into the chemotherapy group with 30 mice and the hormone group with 30 mice according to the serial number. The sperm and spermoblast disappearance situation in the mices testicular seminiferous tubules after the drug injection of the two groups was observed. Results In the chemotherapy group, the mices sperm and spermoblast in the testicular seminiferous tubules disappeared in the 4th week of drug injection,and no sperm was produced in the 20th week of chemotherapy. In the hormone group,after continuous injection of estradiol benzoate,the mices sperm and spermoblast in the testicular seminiferous tubules disappeared completely in the 4th week,and in the 20th week of continuous injection,little spermoblast and sperm was found in the mices testicular seminiferous tubules. The two groups of mice had significantly different testical weight,with statistical significance(P＜0.05). Conclusion The mice azoospermatism animal model established by injecting estradiol benzoate in the hormone group lacks stability.The mice azoospermatism animal model established by injecting busulfan and cyclophosphami