奥曲肽与吉西他滨联合应用对胰腺癌细胞capan-1的影响-普通外科学专业论文.docxVIP

青海大学医学院 青海大学医学院 2010 级研究生硕士论文 第 第 PAGE 5 页 共 30 页 Effects of Octreotide combinated with Gemcitabine in Human pancreatic cancer cell Capan-1 Postgraduate :li Runhua Directed by : prof.QinWei ABSTRACT Objectives Research somatostatin analogue octreotide and DNA nucleotide analogue gemcitabine combined with human pancreatic cancer cells Capan-1 proliferation explore the combined effect between the two drugsexperimental and theoretical basis for the treatment of pancreatic cancer drug. Methods Human pancreatic cancer cells Capan-1 as experimental subjects, divided into four groups, the octreotide group, gemcitabine gemcitabine group and the combination group.CCK-8 assay analysis of different concentrations of octreotide with gemcitabine alone and in combination Capan-1 proliferation of human pancreatic cancer cells; effect principle analysis of the effects of the two drugs in combination. Results 1 .Octreotide (1×10-6 -1×10-1umol/L) and gemcitabine (3×10-8 -3×10-3umol/L) alone and the combination (octreotide 1×10-6 -1×10-1umol/L + gemcitabine 3×10-8 -3×10-3umol/L) inhibited human pancreatic cancer cells Capan-1 growth and proliferation of cells of the combined treatment group compared with the control group and the monotherapy group was significantly reduced relative proliferative capacity and dose-dependent manner, and the difference was statistically significant (P 0.05). 2. Effect principle analysis of the combination of the two drugs effect: the octreotide effect concentration (Dm) 5.2836 ×10-3 umol / L, the gemcitabine in effect concentration (Dm) 3.6543 ×10-5umol / L, the combination effective concentration (Dm) of 3.0154 ×10-4umol / L, the dose of the combination group was significantly lower than the individual dose. Effect principle analysis prompted the two drugs in combination have a synergistic effect (CI 1). Conclusion 1 .Octreotide with gemcitabine alone and in combination are able to inhibit the growth and proliferation of pancreatic cancer cells Capan