芭蕉根和芭蕉花活性成分分析-中药学专业论文.docxVIP

The inhibitory effect against α-glucosidase of different extracts and compounds from rhizomes and flowers of M. basjoo were screened in a 96-well plate. Petroleum ether, EtOAC, MeOH extracts of the rhizomes and petroleum ether extracts of the flowers showed stronger inhibitory effect against a-glucosidase, and petroleum ether extracts of the flowers was the highest. 3,3-bis-hydroxyanigorufone （IC50 = 24.15 μg · mL-1）and 2,4-dihydroxy-9-(4-hydroxyphenyl)-phenalenone（IC50 = 2.81 μg · mL-1） exhibited stronger inhibitory activity ,which were much higher than that of acarbose（IC50 = 1103.01 μg · mL-1）. Antibacterial activity of different extracts and compounds from rhizomes and flowers of M. basjoo were assayed by using Staphylococcus aureus(SA), methicillin-resistant Staphylococcus aureus (MRSA) and β-lactamase positive Staphylococcus aureus(ESBLs-SA) . Petroleum ether extracts of rhizome and flower were active fraction. 2,4-dihydroxy-9-(4-hydroxyphenyl)-phenalenone showed strong activity with MIC value of 0.078, 0.313, 0.039 μg/disc against SA, MRSA and ESBLs-SA respectively. Chapter 2. The study progress of chemical constituent and pharmaceutical of the genus Trapa The study progress of chemical constituent and pharmaceutical of the genus Trapa were summarized. KEY WORDS: Musa basjoo Sieb. et Zucc, chemical constituents, antioxidant, α-glucosidase, antibacterial, research progress IV  第一章 芭蕉根和芭蕉花的化学成分及生物活性研究 第一章 芭蕉根和芭蕉花的化学成分及生物活性研究 第一节 芭蕉根的化学成分研究 1.1 前言 苗药芭蕉(Musa basjoo Sieb. et Zucc) 系芭蕉科芭蕉属植物，多年生草本植物，是热 带的主要果树之一，在我国多分布于秦岭淮河以南地区，贵州、广东、广西、海南、四 川和云南等省均有分布。芭蕉根为芭蕉的干燥根茎，收载于 2003 年版《贵州省中药材·民 族药材质量标准》中，作为苗族的习用药材，具有清热解毒、止渴、利尿等功效，临床 上常用于治疗风热头痛、水肿脚气、血淋、肌肤肿痛、丹毒等[1]。 芭蕉科芭蕉属植物约 40 余种，印度东南至泰国地区种数最多，其次是印度尼西亚 地区。云南连栽培的在内约 9 种，接近我国本属植物的全部种数[2]。查阅相关文献发现， 同属小果野蕉(M. acuminate)、野蕉(M. balbisiana)、大蕉(M. sapientum)和香蕉(M. nana) 报道了 76 个化合物[3-6]，主要化合物类型为 phenylphenalenone、三萜、黄酮和生物碱类。 孙宜春等采用水提取法、醇提取法和石油醚提取法对芭蕉根的化学成分进行了定性试 验，结果表明芭蕉根中含有皂苷、蒽醌、黄酮、香豆素、甾类等成分[7]。未见有对芭蕉 根具体化学成分的报道。 我们对采自贵州省都匀地区的