肺炎支原体肺炎患儿免疫功能临床分析.docVIP

肺炎支原体肺炎患儿免疫功能临床分析 　　[摘要] 目的 研究不同程度的肺炎支原体肺炎患儿T细胞亚群、免疫球蛋白水平变化，探讨其免疫功能以为临床治疗提供依据。 方法 用流式细胞仪检测肺炎支原体肺炎患儿急性期（重症组、普通组）和正常对照组儿童外周血T细胞亚群，用免疫比浊法检测血清IgA、IgM、IgG水平。结果 肺炎支原体肺炎重症组T细胞亚群CD3+、CD4+、CD4+/CD8+低于普通组和对照组（P0.05）。普通组和重症组肺炎血清IgA、IgM、IgG水平均高于正常对照组（P0.01），但IgG重症组低于普通组，差异有统计学意义（P0.01）。 结论 肺炎支原体肺炎患儿存在细胞免疫和体液免疫功能失调，检测T细胞亚群、免疫球蛋白水平，有利于判断肺炎支原体肺炎患儿病情，为临床应用免疫调节剂提供理论依据。 　　[关键词] 肺炎支原体肺炎；T细胞亚群；免疫球蛋白；儿童 　　[中图分类号] R725.6 [文献标识码] B [文章编号] 1673-9701（2014）31-0015-03 　　Clinical significance of immune function of the children with mycoplasma pneumoniae pneumonia 　　LU Xiaoping1 SUN Fei1 YUAN Zhefeng2 　　1.Department of Pediatrics， the First Hospital of Jiaxing City in Zhejiang Province， Jiaxing 314000， China； 2.Children’s Hospital Affiliated to Zhejiang University Medical College， Hangzhou 310003， China 　　[Abstract] Objective To study the levels of T-Lymphocyte subsets and Immunoglobulin of children with varying degrees of mycoplasma pneomoniae pneumonia， discussed the immunity function and provide a basis for clinical treatment. Methods Peripheral blood samples were collected from the children with mycoplasma pneumoniae pneumonia and healthy children. Then T-Lymphocyte subsets were detected by flow cytometry， and the IgA， IgM， IgM and IgG were tested by the immunoturbidimetric assay. Results The percentages of T-Lymphocyte subsets CD3+， CD4+， CD4+/CD8+ in severe mycoplasma pneumoniae pneumonia group were lower than those in control and mild group （P0.05）. The concentrations of IgA， IgM and IgG in sever group and mild group were higher than those in control group （P0.01）. But the percentages of IgG in sever group were lower than those in control， the difference were statistically significant （P0.01）. Conclusion Children with MPP have cellular immune and humoral immune disorders. Through the detection of T-lymphocyte subsets and immunoglobulin， it will be helpful to judge the patients’s condition， which provides a theoretical basis for the clinical application of immune regulators. 　　[K