胆固醇代谢紊乱SD大鼠肝细胞膜生物泵糠蛋白表达平行对照分析.docVIP

胆固醇代谢紊乱SD大鼠肝细胞膜生物泵糠蛋白表达平行对照分析 　　[摘要] 目的 分析?固醇代谢紊乱的SD大鼠肝细胞膜生物泵糖蛋白（Pgp）及相关生化指标与正常大鼠的差异性。 方法 选取SD大鼠30只，随机均分为对照组与观察组，每组各15只；观察组大鼠以高脂饲料喂养法制备为胆固醇代谢紊乱模型，对照组大鼠常规喂养保持健康；以免疫组化法分析两组大鼠肝脏Pgp表达水平并进行比较，同时比较两组大鼠肝匀浆的各项相关生化指标。 结果 对照组大鼠肝脏Pgp平均光度值为（1.01±0.13），肝指数为（1.69±0.68）；观察组大鼠肝脏Pgp平均光度值为（5.36±1.21），肝指数（3.35±0.37）；观察组肝脏Pgp平均光度值与肝指数均显著高于对照组（P0.05）；观察组与对照组大鼠血清Tch比较差异有统计学意义（P0.05）；两组其他各项指标均差异具有统计学意义（P0.01）。肝脏脂肪病变程度与肝脏Pgp光度值呈显著高度正相关性，0.8r1，P0.01。 结论 胆固醇代谢紊乱SD大鼠的肝细胞膜生物泵糖蛋白表达显著高于健康大鼠，提示肝脏Pgp过高表达在胆固醇代谢紊乱的发生发展过程中发挥着重要作用，应引起胆固醇代谢紊乱防治研究的关注。 　　[关键词] 胆固醇代谢紊乱；P-糖蛋白；肝脏；内分泌 　　[中图分类号] R259 [文献标识码] A [文章编号] 2095-0616（2017）07-09-04 　　Parallel control analysis of Biological pump glycoprotein expression of SD rat liver cell membrane with cholesterol metabolism disorder 　　YU Jingxi 　　Department of General Surgery，the Third Peoples Hospital of Huizhou，Huizhou 516002，China 　　[Abstract] Objective To analyze the difference of Biological pump glycoprotein （Pgp） of SD rat liver cell membrane with cholesterol metabolism disorder and its related biochemical indexes and the normal rats. Methods 30 SD rats were selected and randomly divided into observation group and control group，15 rats in each group.The rats in observation group fed with high fat diet were prepared for the disorder of cholesterol metabolism in model，and rats of control group fed healthy.Immunohistochemical method was used to analyze the expression of Pgp in two groups of rats. And they were compared.The related biochemical indexes of rat liver homogenate of the two groups were also compared. Results The average Pgp value of rat liver of control group was （1.01±0.13），the liver index was （1.69±0.68）.The average Pgp value of rat liver of observation group was （5.36±1.21），the liver index was （3.35±0.37）.The average Pgp value of rat liver and liver index in observation group group were significantly higher than those of control group （P0.05）.There was some statistical difference on rats serum Tch between observation group and control group（