艾灸对ra滑膜细胞jak-stat通路负反馈调节因子影响的研究针灸推拿学专业论文.docxVIP

PAGE PAGE 10 Abstract Objective：To observe the effect of moxibustion on J anus kinase-signal transducer and activator of transcription (JAK-STAT) pathway of synovial cells in rheumatoid arthritis (RA) rabbits for revealing the reverse-feedback accommodate mechanism of moxibustion in the treatment of RA. Methods：A total of 42 rabbits (Japanese Big-ear White species) were randomized ( stratified random) into control , model and moxibustion groups with 14 cases in each.RA model was established by injection of Freund’s Complete Adjuvant(FCA0.5 mL/kg)into the animal’s bilateral joint cavities.Moxibustion was applied to bilateral“Shenshu”(BL23),5 cones every time,once daily(except Sundays),3 weeks altogether.The synovial tissue of joint was sampled for analyzing the expression of SOCS1、SOCS3、STAT1、STAT3 with JAK/STAT Pathway with Bicinchoninic acid (BCA )analytical techniques. Results：Before the therapy,the perimeter of left and right knee joint of each group’s rabbit have non-significance difference(P0.05);Compared with normal group,the perimeters of both knee joints in model group increased significantly from the 7th day to 21st day after injection of FCA(P0.01),while compared with model group,those in moxibustion group decreased considerably from the 7th day on ( P 0.05 ) . Compare model group with normal group,SOCS1、SOCS3、STAT1、STAT3of the JAK/STAT pathway has been activated abnormally,and as a result,the expression of these protein signal molecules has been up-regulated（P0.01）;compare moxibustion group with model group, SOCS1、SOCS3、STAT1and STAT3 some anti-inflammatory signal molecules has been down-regulated（P0.05）. Conclusions:Moxibustion plays the anti-inflammatory and detumescence effect in RA synovial cell of experimental rabbits.Moxibustion regulates some relational factors on reverse-feedback accommodate mechanism of the JAK/STAT Pathway.Maybe it is the one of mechanism playing the anti-inflammatory effect by moxibustion. Key word: Moxibustion Rheumato