艾灸加强药液穴位皮肤吸收法阻抑大鼠肺纤维化的治疗效应及其内源性smad7表达研究-针灸推拿学专业论文.docxVIP

成都中医药大学硕士学位论文 成都中医药大学硕士学位论文 PAGE PAGE 1 中文摘要 目的：研究艾灸加强化纤方药液穴位皮肤吸收方法对肺纤维化模型大鼠 SMAD7 蛋白表达和 mRNA 表达的影响，从阻断 TGF-β 传导通路角度探讨其治 疗效应及其阻抑肺纤维化的作用机制。 方法：选用 SD 大鼠以博莱霉素 A5（BLMA5）气管内注入制作大鼠肺纤 维化模型，造模成功 48 只，随机分成 4 组：模型组﹑激素治疗组﹑药灸结合治 疗组﹑艾灸治疗组，每组 12 只，另设空白对照组 12 只。造模 7 天后开始治疗： 药灸结合组根据“以补为通”的治疗原则，采用在“膏肓俞”和“肺俞”穴滴 加化纤方药液再加以艾灸的方法，每次滴加 5 滴化纤方药液，艾灸至穴位皮肤 上的药液干掉为止（约 10 分钟），每日一次，连续治疗一个月；艾灸组只灸其 “膏肓俞”和“肺俞”穴，每日一次，连续治疗一个月；激素治疗组每只腹腔 注射 5mg/kg 泼尼松，每日一次，连续治疗一个月。治疗结束后，应用免疫组化 和原位杂交的方法结合图像分析系统比较各组肺组织 SMAD7 蛋白表达和 mRNA 表达。 结果：药灸结合治疗组﹑艾灸组﹑激素治疗组中 SMAD7 的表达程度均 较模型组明显增多（P﹤0.01），并且其纤维化程度均有减轻，尤以药灸结合治 疗组为优。 结论：药灸结合治疗能在一定程度上更加有效地抑制大鼠肺纤维化的形 成，且其作用机理可能是通过提高 SMAD7 的表达，抑制 TGF-β 的信号传导通 路表达，从而发挥抑制肺纤维化的作用。 关键词：博莱霉素 肺间质纤维化 SMAD7 TGF-β 药灸结合治 疗 ABSTRACT Object: The present study was performed to observe the effects that HuaXianFang liquid is absorbed by the skin of acupoint reinforced by maxibustion on the expression of SMAD7 and its mRNA in rats with pulmonary fibrosis, and to explore the role of TGF-β in the mechanisms underlying . Method: Animal model were successfully obtained by intratracheal injection with BLMA5 in SD rats. 7 days later, rats were randomly divided into 4 groups: model group, prednisone group, herb combined with moxibustion group, and moxibustion group. In addition, 12 healthy rats were set up as contol group. Each group receive corresponding treatment respectively, once daily, 30 consecutive days. Each groups treatment are as follow: model group, no treatment; prednisone group, intraperitoneal injection with prednisone; herb combined with moxibustion group, dropwised by HuaXianFang liquid followed by moxibustion at “GaoHuangShu” and “FeiShu” acupoint; moxibustion group, moxibustion at “GaoHuangShu” and “FeiShu” acupoint. After that, the immunoreactivity of SMAD7 and its mRNA was measured. Results: the expression of SMAD7 in prednisone group, herb combined with moxibustion group, and moxibustion group is significantly increased than model group.all of the three groups have statistical sign