板栗疫病菌自噬相关基因cpatg4的功能研究-生物化学与分子生物学专业论文.docxVIP

广西大学硕士学位论文板栗疫病菌自噬相关基因 广西大学硕士学位论文 板栗疫病菌自噬相关基因 cpatg4 的功能研究 PAGE PAGE 10 Functional Characterizaion of The Autophagy Related Gene cpatg4 in Cryphonectria parasitica ABSTRACT Autophagy is a lysosomal-dependent degradation pathway which is highly conserved in eukaryotrs. Cryphonectria parasitica, as a Pathogenic fungi for chestnut blight, it shows a less pathogenicity when infected by hypovirus, so chestnut trees - chestnut blight fungus - Virus System is considered a excellent material for study in interaction between viruses and fungi and fungal pathogens. The contents of this study include the exploration in the functional Similarity between Cryphonectria parasitica atg4 and yeast gene atg4, and the influence of gene cpatg4 on the Cryphonectria parasitica virulence, sporulation, amount of hypovirus, interaction between between the cpatg4 and cpatg8, and active site of cpatg4. The results were as follows: yeast experiments confirmed that the cpatg4 of Cryphonectria parasitica is presumably homologous to atg4 of yeast in function. After deletion of cpatg4, we found that the Δcpatg4 has a slower growth rate and formed fewer Spore, moreover, virulence seriously decline, but, hypovirus and pigment accumulation are not change.Yeast two-hybrid results indicate that there is interaction between Atg4 protein and Atg8 protein; Point mutations results showed cpatg4 cysteine that have a mutation on 178 amino acids will lead to changes in colony morphology, the Phenomenon predict Cys178 is the point of the active sites of cpatg4. The role of cpatg4 still need further study, and this experiment provide a new way for fungi pathogenesis research. KEY WORDS: Cryphonectria parasitica; autophagy; cpatg4;virulence 目 录 HYPERLINK \l _bookmark0 第一章 前 言 5 HYPERLINK \l _bookmark1 1.1 板栗疫病与板栗疫病菌 5 HYPERLINK \l _bookmark2 1.1.1 板栗疫病 5 HYPERLINK \l _bookmark3 1.1.2 板栗疫病菌 6 HYPERLINK \l _bookmark4 1.1.3 板栗疫病菌低毒力现象与低毒病毒 7 HYPERLINK \l _bookmark5 1.1.4 板栗疫病菌致病机理研究进展 8 HYPER