美托洛尔对心力衰竭大鼠心肌MMP29影响.docVIP

美托洛尔对心力衰竭大鼠心肌MMP29影响 　　 [摘要] 目的:近来研究显示,心力衰竭是心室重塑的过程,其中基质金属蛋白酶2和9(MMP-29)作用最重要,本研究旨在使用美托洛尔干预异丙基肾上腺素(ISO)诱导SD大鼠充血性心力衰竭模型,观察心肌MMP-2 9变化并探讨其意义。方法:实验选用清洁级雄性SD大鼠60只,随机分为正常对照组(n=10)和模型组(n=50)。模型组大鼠皮下注射ISO 2次(170 mg#8226;kg-1),6周后应用超声心动图检测,以左室射血分数≤45%确定造模成功。将心衰大鼠模型随机分为心衰对照组、美托洛尔组(8 mg#8226;kg-1#8226;d-1),18周使用左心导管进行血流动力学检查,取心肌检测心肌MMP-2 9的表达并观察病理形态学特征。结果:(1) 与正常对照组相比,心衰对照组血流动力学指标明显恶化,心室质量指数、左心室质量指数和心肌MMP-2 9表达显著升高(P0.01),心室重构明显;(2) 与心衰对照组比较,美托洛尔组血流动力学指标明显好转,心室质量指数、左心室质量指数和心肌MMP-2 9表达明显降低(P0.01),心室重构好转。结论:美托洛尔能抑制心力衰竭大鼠心肌MMP-2 9表达,改善心功能,逆转心室重构。? 　　[关键词] 美托洛尔; 充血性心力衰竭; 心肌纤维化; 基质金属蛋白酶? 　　[中图分类号] R541.6; R972; R-33[文献标识码] A[文章编号] 1671-7562(2010)01-0019-05? 　　doi:10.3969/j.issn.1671-7562.2010.01.005?? 　　 　　Effect of metoprolol on change of cardiac MMPs-2 9 in rats with ? 　　ISO-induced congestive heart failure?? 　　GUO Han, LU Le, ZHAO Zhi-ming, GUO Jun-hao, ZHAO Ling-jie, CAI Hui 　　(Department of Integrated Traditional Chinese and Western Medicine, Nanjing General Hospital of Nanjing Military Command, PLA, Nanjing 210002, China)?? 　　[Abstract] Objective: Recent studies have suggested that congestive heart failure(CHF) is a myocardial remodeling and lead to the development and progression of CHF. And Matrix metalloproteinase 2 and 9(MMPs-2 9) were the most important factors. This study intends to establish the animal model of isoproterenol(ISO)- induced CHF in rats and to explore the effect of metoprolol on cardiac MMP-2 9. Methods: 60 Male Sprague- Dawley rats were randomly allocated to the ISO-induced chronic heart failure group(n=50) receiving two subcutaneous injections of 170 mg ISO per-kilogram of body and the healthy normal group(n=10) receiving two subcutaneous injections of 0.25 ml normal saline. After 6 weeks, the left ventricular ejection fraction(LVEF) were measured by echocardiogram, the remaining rats, with an LVEF≤45%, were randomly divided into two groups: control group(n=10), metoprolol group 8 mg#8226;kg-1#8226;d-1(n=10), the drugs were given i.g