解聚素―金属蛋白酶17和上皮生长因子受体在人脑胶质瘤中表达及其临床意义.docVIP

解聚素―金属蛋白酶17和上皮生长因子受体在人脑胶质瘤中表达及其临床意义 　　[摘要] 目的 探讨解聚素-金属蛋白酶17（ADAM17）和上皮生长因子受体（EGFR）在人脑胶质瘤的表达及临床意义。 方法 回顾性分析同济大学附属第十人民医院（以下简称“我院”）2011年1月～2013年11月收治的并行外科手术治疗的人脑胶质瘤患者38例，选择同期在我院行外科手术治疗脑外伤患者15例为对照组。采用免疫组织化学法和Western blot法检测胶质瘤组织中ADAM17和EGFR蛋白的表达，并分析其与人脑胶质瘤恶性程度的相关性。 结果 人脑胶质瘤中ADAM17和EGFR表达较对照组明显增多（P 0.05），且随着恶性程度的提高，表达逐渐增多，差异有统计学意义（P 0.05）。 结论 ADAM17和EGFR在人脑胶质瘤细胞内均有表达，且随着肿瘤恶性程度的增高而增加，提示ADAM17和EGFR在人脑胶质瘤的发生过程中可能起重要的作用，其水平高低与胶质瘤的恶性程度具有一定相关性。 　　[关键词] 胶质瘤；解聚素-金属蛋白酶17；表皮生长因子受体 　　[中图分类号] R730 [文献标识码] A [文章编号] 1673-7210（2014）11（a）-0027-04 　　Expression of ADAM17 and EGFR in human brain glioma and its clinical significance 　　SHEN Zhaoli XUE Fei SHEN Rui CHEN Xianzhen 　　Department of Neurosurgery， the 10th Peoples Hospital Affiliated to Tongji University， Shanghai 200072， China 　　[Abstract] Objective To explore the expression of ADAM17 and EGFR in human brain gliomas and its clinical signifcance. Methods 38 patients with human brain glioma treated for surgical operation in the 10th Peoples Hospital Affiliated to Tongji University （our hospital for short） from January 2011 to November 2013 were analyzed retrospectively. 15 patients with cerebral trauma treated for surgical operation in our hospital were selected as the control group. SP immunohistochemical method and Western blot were used to examine the expression of ADAM17， EGFR， and the relationship between the expression of ADAM17， EGFR and pathological grading in human brain glioma was analyzed. Results ADAM17 and EGFR expression in human brain glioma were significantly higher than those in the normal controls （P 0.05）. The expression levels of ADAM17 and EGFR proteins increased with the increased grade of malignancy， and difference was statistically significant （P 0.05）. Conclusion ADAM17 and EGFR are expressed in the human brain glioma. The levels of ADAM17 and EGFR in human brain glioma increased with the rise of tumor grade. The results indicate that ADAM17 and EGFR may play important roles in the occurrence of